Additional support
- Project design and strategy optimisation based on years of experience in creating both simple and complex alleles.
- Supply/production of relevant reagents.
- Choice of a broad range of techniques, according to the required genetic alteration. These include classical gene targeting, genome editing using CRISPR/Cas9 in early embryos, CRISPR-aided targeting in embryonic stem cells, or additive transgenesis to generate the validated mouse.
- Allele types include tags, knock-ins, reporter and recombinase expressors, humanisation, inducible overexpression systems, large-scale deletions or duplication.
- Full validation of the genetic sequence of the mutant allele.
- Further functional validation of transgenes. This includes the option of mutant transcript quantification and recombinase activity profiling.
- We also offer the characterisation of copy number and point of insertion of transgenes obtained by additive transgenesis.