B6.Cg-Rasgrf1^tm1Kln/Cnrm

Status	Available to order
EMMA ID	EM:10171
International strain name	B6.Cg-Rasgrf1^tm1Kln/Cnrm
Alternative name	Rasgrf1 KO
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Rasgrf1^tm1Kln,
Gene/Transgene symbol	Rasgrf1

Information from provider

Provider	Riccardo Brambilla
Provider affiliation	School of Biosciences, Cardiff University
Genetic information	Genetic inactivation via Homologous Recombination in ES cells of the Rasgrf1 locus. Exon 3-5 were replaced by a Neomycin Cassette.
Phenotypic information	Homozygous: Ras-GRF1 KO mice showed learning and deficit phenotypes, and alterations of the responses to drugs of abuse. Moreover they showed altered response to L-DOPA induced dyskinesia. Mutant mice are also smaller (10% reduction of body weight) than the littermate control. Heterozygous: The RasGRF1 locus is genetically imprinted (paternal allele-specific expression). Therefore, depending on the breeding protocol, some heterozygous mice are functionally KO.
Breeding history	Animals were generated at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany, in 1995 and then transferred in 1998 to the San Raffaele Scientific Institute in Milan, Italy
References	A role for the Ras signalling pathway in synaptic transmission and long-term memory.;Brambilla R, Gnesutta N, Minichiello L, White G, Roylance A J, Herron C E, Ramsey M, Wolfer D P, Cestari V, Rossi-Arnaud C, Grant S G, Chapman P F, Lipp H P, Sturani E, Klein R, ;1997;Nature;390;281-6; 9384379 Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) controls activation of extracellular signal-regulated kinase (ERK) signaling in the striatum and long-term behavioral responses to cocaine.;Fasano Stefania, D'Antoni Angela, Orban Paul C, Valjent Emmanuel, Putignano Elena, Vara Hugo, Pizzorusso Tommaso, Giustetto Maurizio, Yoon Bongjune, Soloway Paul, Maldonado Rafael, Caboche Jocelyne, Brambilla Riccardo, ;2009;Biological psychiatry;66;758-68; 19446794 Inhibition of Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) signaling in the striatum reverts motor symptoms associated with L-dopa-induced dyskinesia.;Fasano Stefania, Bezard Erwan, D'Antoni Angela, Francardo Veronica, Indrigo Marzia, Qin Li, Doveró Sandra, Cerovic Milica, Cenci M Angela, Brambilla Riccardo, ;2010;Proceedings of the National Academy of Sciences of the United States of America;107;21824-9; 21115823 Mice Lacking Ras-GRF1 Show Contextual Fear Conditioning but not Spatial Memory Impairments: Convergent Evidence from Two Independently Generated Mouse Mutant Lines.;d'Isa Raffaele, Clapcote Steven J, Voikar Vootele, Wolfer David P, Giese Karl Peter, Brambilla Riccardo, Fasano Stefania, ;2011;Frontiers in behavioral neuroscience;5;78; 22164138 Derangement of Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) and extracellular signal-regulated kinase (ERK) dependent striatal plasticity in L-DOPA-induced dyskinesia.;Cerovic Milica, Bagetta Vincenza, Pendolino Valentina, Ghiglieri Veronica, Fasano Stefania, Morella Ilaria, Hardingham Neil, Heuer Andreas, Papale Alessandro, Marchisella Francesca, Giampà Carmela, Calabresi Paolo, Picconi Barbara, Brambilla Riccardo, ;2015;Biological psychiatry;77;106-15; 24844602 Differential involvement of Ras-GRF1 and Ras-GRF2 in L-DOPA-induced dyskinesia.;Bido Simone, Solari Nicola, Indrigo Marzia, D'Antoni Angela, Brambilla Riccardo, Morari Michele, Fasano Stefania, ;2015;Annals of clinical and translational neurology;2;662-78; 26125041
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

MGI phenotypes (allele matching)

reduced long term potentiation / MGI
impaired synaptic plasticity / MGI
abnormal active avoidance behavior / MGI
abnormal excitatory postsynaptic potential / MGI
nervous system phenotype / MGI
behavior/neurological phenotype / MGI
abnormal long term spatial reference memory / MGI
impaired contextual conditioning behavior / MGI

Literature references

A role for the Ras signalling pathway in synaptic transmission and long-term memory.;Brambilla R, Gnesutta N, Minichiello L, White G, Roylance A J, Herron C E, Ramsey M, Wolfer D P, Cestari V, Rossi-Arnaud C, Grant S G, Chapman P F, Lipp H P, Sturani E, Klein R, ;1997;Nature;390;281-6; 9384379
Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) controls activation of extracellular signal-regulated kinase (ERK) signaling in the striatum and long-term behavioral responses to cocaine.;Fasano Stefania, D'Antoni Angela, Orban Paul C, Valjent Emmanuel, Putignano Elena, Vara Hugo, Pizzorusso Tommaso, Giustetto Maurizio, Yoon Bongjune, Soloway Paul, Maldonado Rafael, Caboche Jocelyne, Brambilla Riccardo, ;2009;Biological psychiatry;66;758-68; 19446794
Inhibition of Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) signaling in the striatum reverts motor symptoms associated with L-dopa-induced dyskinesia.;Fasano Stefania, Bezard Erwan, D'Antoni Angela, Francardo Veronica, Indrigo Marzia, Qin Li, Doveró Sandra, Cerovic Milica, Cenci M Angela, Brambilla Riccardo, ;2010;Proceedings of the National Academy of Sciences of the United States of America;107;21824-9; 21115823
Mice Lacking Ras-GRF1 Show Contextual Fear Conditioning but not Spatial Memory Impairments: Convergent Evidence from Two Independently Generated Mouse Mutant Lines.;d'Isa Raffaele, Clapcote Steven J, Voikar Vootele, Wolfer David P, Giese Karl Peter, Brambilla Riccardo, Fasano Stefania, ;2011;Frontiers in behavioral neuroscience;5;78; 22164138
Derangement of Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) and extracellular signal-regulated kinase (ERK) dependent striatal plasticity in L-DOPA-induced dyskinesia.;Cerovic Milica, Bagetta Vincenza, Pendolino Valentina, Ghiglieri Veronica, Fasano Stefania, Morella Ilaria, Hardingham Neil, Heuer Andreas, Papale Alessandro, Marchisella Francesca, Giampà Carmela, Calabresi Paolo, Picconi Barbara, Brambilla Riccardo, ;2015;Biological psychiatry;77;106-15; 24844602
Differential involvement of Ras-GRF1 and Ras-GRF2 in L-DOPA-induced dyskinesia.;Bido Simone, Solari Nicola, Indrigo Marzia, D'Antoni Angela, Brambilla Riccardo, Morari Michele, Fasano Stefania, ;2015;Annals of clinical and translational neurology;2;662-78; 26125041

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.

B6.Cg-Rasgrf1tm1Kln/Cnrm