C57BL/6NTac-Nodal^{tm1a(EUCOMM)Wtsi}/H

Status	Available to order
EMMA ID	EM:10453
Citation information	RRID:IMSR_EM:10453 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6NTac-Nodal^{tm1a(EUCOMM)Wtsi}/H
Alternative name	EPD0197_2_B12
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Nodal^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Nodal
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone EPD0197_2_B12. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6NTac males
Breeding at archiving centre	This mutation was made on a coisogenic C57BL/6NTac background

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Situs inversus totalis / Orphanet_101063
Alobar holoprosencephaly / Orphanet_93925
Midline interhemispheric variant of holoprosencephaly / Orphanet_93926
Microform holoprosencephaly / Orphanet_280200
Septopreoptic holoprosencephaly / Orphanet_280195
Semilobar holoprosencephaly / Orphanet_220386
Lobar holoprosencephaly / Orphanet_93924

IMPC phenotypes (gene matching)

corneal vascularization / IMPC
preweaning lethality, complete penetrance / IMPC
increased circulating HDL cholesterol level / IMPC
embryonic lethality prior to tooth bud stage / IMPC
decreased locomotor activity / IMPC
increased grip strength / IMPC
increased circulating triglyceride level / IMPC
abnormal startle reflex / IMPC
increased large unstained cell number / IMPC
increased circulating cholesterol level / IMPC

MGI phenotypes (gene matching)

abnormal heart development / MGI
abnormal heart looping / MGI
abnormal interventricular septum morphology / MGI
enlarged pericardium / MGI
trabecula carnea hypoplasia / MGI
abnormal foregut morphology / MGI
right-sided isomerism / MGI
right pulmonary isomerism / MGI
abnormal liver morphology / MGI
small liver / MGI
pale liver / MGI
abnormal spleen morphology / MGI
small spleen / MGI
spleen hypoplasia / MGI
abnormal brain development / MGI
absent floor plate / MGI
absent notochord / MGI
decreased body size / MGI
abnormal ectoderm development / MGI
failure of primitive streak formation / MGI
abnormal gastrulation / MGI
decreased embryo size / MGI
abnormal embryo turning / MGI
abnormal left-right axis patterning / MGI
absent amniotic folds / MGI
embryonic growth arrest / MGI
pericardial edema / MGI
premature death / MGI
abnormal developmental patterning / MGI
abnormal embryonic tissue morphology / MGI
abnormal extraembryonic tissue morphology / MGI
no abnormal phenotype detected / MGI
abnormal gastrulation movements / MGI
abnormal primitive streak formation / MGI
abnormal primitive streak morphology / MGI
small ectoplacental cone / MGI
situs ambiguus / MGI
aortic hypertrophy / MGI
no phenotypic analysis / MGI
absent allantois / MGI
abnormal digestive system development / MGI
absent placental labyrinth / MGI
abnormal craniofacial development / MGI
thin myocardium compact layer / MGI
abnormal primitive node morphology / MGI
transposition of great arteries / MGI
heterotaxia / MGI
abnormal direction of heart looping / MGI
abnormal spongiotrophoblast layer morphology / MGI
abnormal embryonic neuroepithelium morphology / MGI
abnormal prechordal plate morphology / MGI
absent prechordal plate / MGI
truncated notochord / MGI
abnormal neural fold formation / MGI
absent amnion / MGI
holoprosencephaly / MGI
abnormal rostral-caudal axis patterning / MGI
embryo phenotype / MGI
right atrial isomerism / MGI
abnormal heart position or orientation / MGI
abnormal extraembryonic mesoderm development / MGI
absent somites / MGI
abnormal anterior visceral endoderm morphology / MGI
abnormal hindgut morphology / MGI
abnormal mesendoderm development / MGI
absent primitive node / MGI
increased trophoblast giant cell number / MGI
abnormal lateral plate mesoderm morphology / MGI
atrial septal defect / MGI
common atrium / MGI
abnormal extraembryonic ectoderm morphology / MGI
abnormal stomach position or orientation / MGI
neonatal lethality, complete penetrance / MGI
perinatal lethality, complete penetrance / MGI
prenatal lethality, complete penetrance / MGI
embryonic lethality between implantation and somite formation, complete penetrance / MGI
embryonic lethality between somite formation and embryo turning, complete penetrance / MGI
prenatal lethality, incomplete penetrance / MGI
embryonic lethality, incomplete penetrance / MGI
embryonic lethality during organogenesis, incomplete penetrance / MGI
abnormal visceral endoderm morphology / MGI
abnormal parietal yolk sac morphology / MGI
absent visceral yolk sac / MGI
thin placenta labyrinth / MGI
fused somites / MGI
absent heart tube / MGI
delayed heart development / MGI
absent foregut / MGI
truncated foregut / MGI
absent midbrain / MGI
increased spongiotrophoblast size / MGI
embryonic-extraembryonic boundary constriction / MGI
absent forebrain / MGI
rostral body truncation / MGI
abnormal head development / MGI
abnormal heart apex morphology / MGI
decreased embryonic neuroepithelial cell proliferation / MGI
absent head fold / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

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Legally binding conditions for the transfer

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C57BL/6NTac-Nodaltm1a(EUCOMM)Wtsi/H