129S5;B6N-Caprin1^{tm3a(EUCOMM)Wtsi}/WtsiPh

Status	Available to order
EMMA ID	EM:10989
Citation information	RRID:IMSR_EM:10989 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	129S5;B6N-Caprin1^{tm3a(EUCOMM)Wtsi}/WtsiPh
Alternative name	EPD0989_2_A03
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Caprin1^{tm3a(EUCOMM)Wtsi}
Gene/Transgene symbol	Caprin1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0989_2_A03. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Targeted deletion of the RNA-binding protein Caprin1 leads to progressive hearing loss and impairs recovery from noise exposure in mice.;Nolan Lisa S, Chen Jing, Gonçalves Ana-Cláudia, Bullen Anwen, Towers Emily R, Steel Karen P, Dawson Sally J, Gale Jonathan E, ;2022;Scientific reports;12;2444; 35165318

Information from EMMA

Archiving centre	Institute of Molecular Genetics, Prague, Czech Republic

Disease and phenotype information

MGI phenotypes (gene matching)

abnormal posture / MGI
cyanosis / MGI
respiratory failure / MGI
increased neuron apoptosis / MGI
decreased fetal size / MGI
abnormal miniature excitatory postsynaptic currents / MGI
abnormal neuron physiology / MGI
abnormal dendrite morphology / MGI
abnormal synapse morphology / MGI
neonatal lethality, complete penetrance / MGI

Literature references

Targeted deletion of the RNA-binding protein Caprin1 leads to progressive hearing loss and impairs recovery from noise exposure in mice.;Nolan Lisa S, Chen Jing, Gonçalves Ana-Cláudia, Bullen Anwen, Towers Emily R, Steel Karen P, Dawson Sally J, Gale Jonathan E, ;2022;Scientific reports;12;2444; 35165318

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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129S5;B6N-Caprin1tm3a(EUCOMM)Wtsi/WtsiPh