| Status | Available to order |
| EMMA ID | EM:11255 |
| International strain name | B6.129-Wnt11tm1Amc/Oulu |
| Alternative name | Wnt11 knock-out |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Wnt11tm1Amc, |
| Gene/Transgene symbol | Wnt11 |
| Provider | Seppo Vainio |
| Provider affiliation | Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, University of Oulu |
| Additional owner | Andrew McMahon, University of Southern California, US |
| Genetic information | The gene was disrupted by replacement of exons 4 and 5 with a PGK-neo cassette via homologous recombination. RT-PCR analysis of RNA from homozygous mutant animals revealed a truncated transcription product predicted to encode 28 N-terminal amino acids. This mutant protein is expected to be non-functional. |
| Phenotypic information | Homozygous:Mortality/aging: The presence of the Wnt11 null allele in the C57BL/6 background partially overcomes the in utero lethality reported in the SV129 background. 1/3 of the Wnt11-/- mice with this inbred background survives to postnatal stages. Many of them also reach adulthood. The number of the surviving Wnt11-/- mice drops by around 50% by the age of 4–6 weeks. An additional reduction of 10% takes place by the age of 12 months. The proportion of female to male C57BL/6 Wnt11-/- survivors remains unchanged. Renal/urinary system: Hypoplastic kidneys. Glomerular cysts. Reduced amount of glomeruli. Compromised kidney function.Heterozygous:Mice heterozygous for this targeted mutation are viable and fertile with normal kidney size and histology. |
| Breeding history | A targeting vector was designed to replace exons 4 and 5 of the endogenous gene with a PGK-neo cassette. This construct was microinjected into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Chimeric mice were bred to "129 substrain AV3" inbred mice (129X1/SvJ). Heterozygous mice were maintained and expanded on this same genetic background (129X1/SvJ) prior to crossing to C57BL/6JOlaHsd and from 2014 to C57BL/6N (5 generations). Totally over 10 generations in C57 background. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | University of Oulu, Oulu, Finland |
| Animals used for archiving | heterozygous C57BL/6NCrl |
