C57BL/6N-A^tm1Brd Kifap3^{tm1a(KOMP)Wtsi}/WtsiOulu

Status	Available to order
EMMA ID	EM:11555
Citation information	RRID:IMSR_EM:11555 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-A^tm1Brd Kifap3^{tm1a(KOMP)Wtsi}/WtsiOulu
Alternative name	EPD0320_1_E08
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Kifap3^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Kifap3
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0320_1_E08. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland

Disease and phenotype information

IMPC phenotypes (allele matching)

impaired glucose tolerance / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

impaired glucose tolerance / IMPC
preweaning lethality, complete penetrance / IMPC

MGI phenotypes (gene matching)

abnormal heart morphology / MGI
enlarged heart / MGI
abnormal thymus morphology / MGI
small thymus / MGI
decreased thymocyte number / MGI
weakness / MGI
abnormal medulla oblongata morphology / MGI
abnormal dorsal root ganglion morphology / MGI
abnormal trigeminal ganglion morphology / MGI
cyanosis / MGI
edema / MGI
abnormal myocardial trabeculae morphology / MGI
increased liver weight / MGI
abnormal heart atrium morphology / MGI
increased cardiomyocyte apoptosis / MGI
decreased skin turgor / MGI
abnormal heart ventricle morphology / MGI
abnormal myocardium layer morphology / MGI
increased lung weight / MGI
abnormal heart septum morphology / MGI
increased thymocyte apoptosis / MGI
pulmonary vascular congestion / MGI
liver vascular congestion / MGI
postnatal lethality, incomplete penetrance / MGI
increased spinal cord apoptosis / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Atm1Brd Kifap3tm1a(KOMP)Wtsi/WtsiOulu