C57BL/6N-A^tm1Brd Gas2l2^{tm1a(KOMP)Wtsi}/WtsiOulu

Status	Available to order
EMMA ID	EM:11597
Citation information	RRID:IMSR_EM:11597 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-A^tm1Brd Gas2l2^{tm1a(KOMP)Wtsi}/WtsiOulu
Alternative name	EPD0626_4_G11
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Gas2l2^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Gas2l2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0626_4_G11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Primary ciliary dyskinesia / Orphanet_244

IMPC phenotypes (allele matching)

male infertility / IMPC
female infertility / IMPC
abnormal auditory brainstem response / IMPC
absent pinna reflex / IMPC
decreased total body fat amount / IMPC

IMPC phenotypes (gene matching)

absent pinna reflex / IMPC
abnormal auditory brainstem response / IMPC
decreased total body fat amount / IMPC
male infertility / IMPC
female infertility / IMPC

MGI phenotypes (allele matching)

abnormal basisphenoid bone morphology / MGI
abnormal vertebral body morphology / MGI
abnormal snout morphology / MGI
enlarged liver sinusoidal spaces / MGI
absent salivary gland / MGI
abnormal forebrain morphology / MGI
abnormal midbrain morphology / MGI
incomplete rostral neuropore closure / MGI
fused dorsal root ganglion / MGI
anophthalmia / MGI
aphakia / MGI
biliary cyst / MGI
abnormal auditory tube / MGI
dilated ureter / MGI
abnormal eye muscle morphology / MGI
abnormal optic stalk morphology / MGI
absent vertebral arch / MGI
fusion of vertebral arches / MGI
absent stapedial artery / MGI
abnormal Meckel's cartilage morphology / MGI
oral atresia / MGI
absent tongue / MGI
perimembraneous ventricular septal defect / MGI
absent optic cup / MGI
absent segment of anterior cerebral artery / MGI
abnormal Mullerian duct topology / MGI
trigeminal neuroma / MGI
abnormal ductus venosus valve morphology / MGI
abnormal thymus topology / MGI
abnormal left vena cava superior connection / MGI
abnormal external carotid artery origin / MGI
abnormal vertebral artery topology / MGI
embryo tumor / MGI

MGI phenotypes (gene matching)

abnormal basisphenoid bone morphology / MGI
abnormal vertebral body morphology / MGI
abnormal snout morphology / MGI
enlarged liver sinusoidal spaces / MGI
absent salivary gland / MGI
abnormal forebrain morphology / MGI
abnormal midbrain morphology / MGI
incomplete rostral neuropore closure / MGI
fused dorsal root ganglion / MGI
anophthalmia / MGI
no abnormal phenotype detected / MGI
aphakia / MGI
biliary cyst / MGI
abnormal auditory tube / MGI
dilated ureter / MGI
abnormal eye muscle morphology / MGI
abnormal optic stalk morphology / MGI
absent vertebral arch / MGI
fusion of vertebral arches / MGI
absent stapedial artery / MGI
abnormal Meckel's cartilage morphology / MGI
oral atresia / MGI
absent tongue / MGI
perimembraneous ventricular septal defect / MGI
absent optic cup / MGI
absent segment of anterior cerebral artery / MGI
abnormal Mullerian duct topology / MGI
trigeminal neuroma / MGI
abnormal ductus venosus valve morphology / MGI
abnormal thymus topology / MGI
abnormal left vena cava superior connection / MGI
abnormal external carotid artery origin / MGI
abnormal vertebral artery topology / MGI
embryo tumor / MGI
abnormal middle cerebral artery origin / MGI
abnormal ophthalmic artery origin / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Atm1Brd Gas2l2tm1a(KOMP)Wtsi/WtsiOulu