BD2F1-Tg(BKV-HIV/tat)#/Cnrm
| Status | Available to order |
| EMMA ID | EM:11620 |
| International strain name | BD2F1-Tg(BKV-HIV/tat)#/Cnrm |
| Alternative name | BKV/ tat transgenic BDF mice |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(BKV-HIV/tat)# |
| Gene/Transgene symbol | Tg(BKV-HIV-tat)# |
Information from provider
| Provider | Antonella Caputo |
| Provider affiliation | University of Padova |
| Genetic information | To generate transgenic mouse lines carrying BKV/tat-sequences, the linear vector DNA was then microinjected into the pronuclei of fertilized eggs from superovulated BDF1 female mice. BDF1 mice are a hybrid strain obtained by crossing C57BL/6 females with DBA/2 male mice. The hybridization profile is suggestive of a single integration site containing multiple copies of the transgene in a tandem array. The transgene DNA was detected in brain, kidney, thymus, skin, stomach, intestine, lung, liver, and spleen of animals in all mouse lines as tandem insertions in a variable number of copies (5 to 20)/cell. |
| Phenotypic information | Homozygous:Transgenic mice carrying a recombinant DNA containing BK virus early region and the human immunodeficiency virus tat gene can develop skin leiomyosarcomas, squamous cell papillomas and carcinomas, adenocarcinomas of skin adnexa, glands, and B-cell lymphomas. The incidence of hepatocellular carcinoma is low but most animals show a liver cell dysplasia of variable degree. Some of these mice are also affected by skin lesions resembling the early stages of Kaposi's sarcoma. This transgenic mouse model is representative of the systemic involvement of tat in human immunodeficiency virus natural infection and may be applied to investigate the role of tat in malignancies associated to AIDS, to study Kaposi's sarcoma pathogenesis, to characterize tumors, hyperplastic and dysplastic lesions, and non-neoplastic lesions established by tat in the skin and liver, and to assess in vivo the efficacy of antiangiogenic and anti-tat-specific drugs. Moreover these mice display a latent epileptic phenotype, indeed they are highly susceptible to the effect of kainate and are predisposed to develop convulsive seizures leading to cell damage. The HIV-1 Tat protein may play an important role in HIV-1 associated neurocognitive disorders (HAND) and the Tg tat mice could represent a useful model to investigate all the comorbidities in the development of HAND.Heterozygous:No data with these kind of mice. |
| Breeding history | Only backcrossing with BDF1 Charles River |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | not known |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Systemic expression of HIV-1 tat gene in transgenic mice induces endothelial proliferation and tumors of different histotypes.;Corallini A, Altavilla G, Pozzi L, Bignozzi F, Negrini M, Rimessi P, Gualandi F, Barbanti-Brodano G, ;1993;Cancer research;53;5569-75; 8221699
- Promotion of tumour metastases and induction of angiogenesis by native HIV-1 Tat protein from BK virus/tat transgenic mice.;Corallini A, Campioni D, Rossi C, Albini A, Possati L, Rusnati M, Gazzanelli G, Benelli R, Masiello L, Sparacciari V, Presta M, Mannello F, Fontanini G, Barbanti-Brodano G, ;1996;AIDS (London, England);10;701-10; 8805860
- Morphological, histochemical, immunohistochemical, and ultrastructural characterization of tumors and dysplastic and non-neoplastic lesions arising in BK virus/tat transgenic mice.;Altavilla G, Trabanelli C, Merlin M, Caputo A, Lanfredi M, Barbanti-Brodano G, Corallini A, ;1999;The American journal of pathology;154;1231-44; 10233861
- Prevalence of liver tumours in HIV-1 tat-transgenic mice treated with urethane.;Altavilla G, Caputo A, Trabanelli C, Brocca Cofano E, Sabbioni S, Menegatti M A, Barbanti-Brodano G, Corallini A, ;2004;European journal of cancer (Oxford, England : 1990);40;275-83; 14728943
- Increased excitability in tat-transgenic mice: role of tat in HIV-related neurological disorders.;Zucchini Silvia, Pittaluga Anna, Brocca-Cofano Egidio, Summa Maria, Fabris Marina, De Michele Rita, Bonaccorsi Angela, Busatto Graziella, Barbanti-Brodano Giuseppe, Altavilla Giuseppe, Verlengia Gianluca, Cifelli Pierangelo, Corallini Alfredo, Caputo Antonella, Simonato Michele, ;2013;Neurobiology of disease;55;110-9; 23454193