C57BL/6-Dusp9tm2.1Skey/H
| Status | Available to order |
| EMMA ID | EM:11824 |
| International strain name | C57BL/6-Dusp9tm2.1Skey/H |
| Alternative name | DUSP9fl |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Dusp9tm2.1Skey, |
| Gene/Transgene symbol | Dusp9 |
Information from provider
| Provider | Stephen Keyse |
| Provider affiliation | Division of Cancer Research, Ninewells Hospital & Medical School, University of Dundee |
| Genetic information | The murine Dusp9 locus was targeted by the introduction of loxP sites flanking exons 3 and 4 and the insertion of a positive selection marker (puromycin resistance) flanked by Frt sites downstream of exon 4 and immediately 5' to the loxP site within the 3' untranslated region. After electroporation into Art B6/3.5 (C57BL/6NTac) ES cells and puromycin selection, clones carrying the correctly targeted allele were identified and used to generate chimeric mice. Chimeric mice were then crossed with the C57BL/6-Tg(CAG-Flpe)2Arte Flp-deleter strain, resulting in recombination and removal of the puromycin selection marker to generate the conditional allele (Dusp9-fl). |
| Phenotypic information | Homozygous:None detectedHeterozygous:None detected |
| Breeding history | Between 2012 and 2014 DUSP9-fl mice have been backcrossed to C57BL/6J wild-type mice for 7 generations. Since 2014 there has been no backcrossing, just random matings from within the colony. No direct sibling matings are recorded, but the colony is very small (only 2 pairs or trios) so randomising the breeding to avoid pairing closely related animals would not have been possible. There have been 13 generations of these matings. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Selective Expression of the MAPK Phosphatase Dusp9/MKP-4 in Mouse Plasmacytoid Dendritic Cells and Regulation of IFN-β Production.;Niedzielska Magdalena, Raffi Faizal A M, Tel Jurjen, Muench Sandra, Jozefowski Katrin, Alati Nour, Lahl Katharina, Mages Jörg, Billmeier Ulrike, Schiemann Matthias, Appelt Uwe K, Wirtz Stefan, Sparwasser Tim, Hochrein Hubertus, Figdor Carl G, Keyse Stephen M, Lang Roland, ;2015;Journal of immunology (Baltimore, Md. : 1950);195;1753-62; 26170386