C57BL/6-Khdrbs1tm1a(EUCOMM)Hmgu/Orl

Status

Available to order

EMMA IDEM:11963
Citation informationRRID:IMSR_EM:11963 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6-Khdrbs1tm1a(EUCOMM)Hmgu/Orl
Alternative nameB6-Khdrbs1tm1a(EUCOMM)Hmgu
Strain typeTargeted Mutant Strains
Allele/Transgene symbolKhdrbs1tm1a(EUCOMM)Hmgu
Gene/Transgene symbolKhdrbs1

Information from provider

ProviderFrederic Fiore
Provider affiliationCIPHE
Genetic informationEUCOMM knock-out first allele with conditional potential (conditional-ready) of the Khdrbs1 gene. The L1L2_Bact_P cassette was inserted at position 129730797 of Chromosome 4 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 129729969. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knock-out mouse. If cre expression occurs without flp expression, a reporter knock-out mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.
Phenotypic informationHomozygous breeding is difficult because of infertility, even with crosses between heterozygous and homozygous animals. Strain maintained by heterozygous versus heterozygous mating.
ReferencesNone available
Homozygous fertileno
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivinghomozygous C57BL/6J males

Disease and phenotype information

MGI phenotypes (gene matching)
  • increased bone mineral density / MGI
  • abnormal bone marrow development / MGI
  • decreased body weight / MGI
  • abnormal maternal nurturing / MGI
  • abnormal skeleton physiology / MGI
  • male infertility / MGI
  • neoplasm / MGI
  • abnormal bone remodeling / MGI
  • abnormal osteoblast physiology / MGI
  • increased bone mass / MGI
  • abnormal adipose tissue physiology / MGI
  • decreased circulating leptin level / MGI
  • reduced cerebellar foliation / MGI
  • perinatal lethality, incomplete penetrance / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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