| Status | Available to order |
| EMMA ID | EM:12199 |
| Citation information | RRID:IMSR_EM:12199 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;D2-Mettl16em1Embrp/Orl |
| Alternative name | Mettl16+/-, C57Bl6 background; Mettl16-em1Rspi |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Mettl16em1Embrp |
| Gene/Transgene symbol | Mettl16 |
| Provider | Ramesh PILLAI |
| Provider affiliation | Molecular Biology, University of Geneva |
| Genetic information | The mutant is a knock-out allele of the Mettl16 gene. It was obtained by insertion of a triple-stop cassette into exon 3 of the Mettl16 gene locus. The Mettl16 gene locus is located on mouse chromosome 11 and consists of 10 exons. We targeted the endogenous Mettl16 locus in mouse embryos of the B6D2F1/J hybrid line (also called B6D2; The Jackson Laboratory, stock no. 100006). It is a cross between C57BL/6J (B6) and DBA/2J (D2), and heterozygous for all B6 and D2 alleles. Single-cell mouse embryos were injected with a guide RNA (gRNA) that directs the DNA endonuclease Cas9, and a 170 nt single-stranded DNA (ssDNA) repair template (IDT). The ssDNA carries a triple-stop codon flanked by a 81 nt 5 prime homology arm and a 75 nt 3 prime homology arm. Founder mice were identified by genotyping PCR and crossed with wild-type C57BL/6JRj (Janvier) partners to obtain germline transmission. We obtained a line where the homology recombination template was inserted, resulting in a triple-stop codon cassette (sequence: ATGTAAATAGATGA) in exon 3. |
| Phenotypic information | Homozygous:Homozygous Mettl16 mice do not survive beyond embryonic day 3.5 (E3.5). Embryonic lethality around implantation stage.Heterozygous:Heterozygous +/- male and female mice are perfectly normal in terms of viability and fertility. |
| Breeding history | The initial F1 obtained were crossed either with C57BL/6JRj wild-type mice or with F1 siblings to maintain the line. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | not known |
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J males |
