C57BL/6N-Atm1Brd Sik3tm1a(EUCOMM)Hmgu/WtsiOrl

Status

Available to order

EMMA IDEM:12432
International strain nameC57BL/6N-Atm1Brd Sik3tm1a(EUCOMM)Hmgu/WtsiOrl
Alternative nameHEPD0744_6_B02
Strain typeTargeted Mutant Strains
Allele/Transgene symbolSik3tm1a(EUCOMM)Hmgu,
Gene/Transgene symbolSik3
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone HEPD0744_6_B02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

IMPC phenotypes (allele matching)
  • abnormal vertebrae morphology / IMPC
  • abnormal rib morphology / IMPC
  • kyphosis / IMPC
  • decreased circulating calcium level / IMPC
  • increased neutrophil cell number / IMPC
  • increased granulocyte number / IMPC
  • abnormal cranium morphology / IMPC
  • abnormal radius morphology / IMPC
  • abnormal tibia morphology / IMPC
  • abnormal femur morphology / IMPC
  • decreased body length / IMPC
  • abnormal cornea morphology / IMPC
  • decreased caudal vertebrae number / IMPC
  • female infertility / IMPC
  • abnormal coat/hair pigmentation / IMPC
  • abnormal tail morphology / IMPC
  • abnormal fibula morphology / IMPC
  • decreased circulating triglyceride level / IMPC
  • decreased circulating free fatty acid level / IMPC
  • decreased circulating insulin level / IMPC
  • decreased erythrocyte cell number / IMPC
  • abnormal joint morphology / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • thrombocytopenia / IMPC
  • abnormal bone structure / IMPC
  • increased lean body mass / IMPC
  • decreased lean body mass / IMPC
  • abnormal spine curvature / IMPC
  • abnormal pelvic girdle bone morphology / IMPC
  • increased eosinophil cell number / IMPC
  • decreased T cell number / IMPC
  • abnormal ulna morphology / IMPC
  • abnormal zygomatic bone morphology / IMPC
  • improved glucose tolerance / IMPC
  • abnormal humerus morphology / IMPC
  • increased circulating bilirubin level / IMPC
  • decreased circulating glucose level / IMPC
  • increased mean corpuscular hemoglobin / IMPC
  • decreased blood urea nitrogen level / IMPC
  • decreased circulating potassium level / IMPC
  • increased circulating sodium level / IMPC
  • decreased NK T cell number / IMPC
  • increased NK cell number / IMPC
  • decreased NK cell number / IMPC
  • decreased CD4-positive, alpha beta T cell number / IMPC
  • increased CD8-positive, alpha-beta T cell number / IMPC
  • increased follicular B cell number / IMPC
  • decreased germinal center B cell number / IMPC
  • decreased gamma-delta T cell number / IMPC
  • increased plasmacytoid dendritic cell number / IMPC
  • decreased total body fat amount / IMPC
  • increased red blood cell distribution width / IMPC
  • decreased circulating fructosamine level / IMPC
  • abnormal thoracic cage shape / IMPC
  • increased bone mineral content / IMPC
  • increased CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
  • decreased alpha-beta T cell number / IMPC
  • decreased KLRG1-positive NK cell number / IMPC
  • increased Ly6C high monocyte number / IMPC
  • decreased Ly6C low monocyte number / IMPC
  • increased KLRG1+ CD8 alpha beta T cell number / IMPC
  • increased memory-marker gamma-delta T cell number / IMPC
  • decreased CD5-positive gamma-delta T cell number / IMPC
  • increased memory-marker NK cell number / IMPC
  • increased KLRG1-positive CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
  • increased CD8-positive, naive alpha-beta T cell number / IMPC
  • increased CD4-negative NK T cell number / IMPC
  • decreased memory-marker CD4-negative NK T cell number / IMPC
  • increased KLRG1-positive CD4-negative NK T cell number / IMPC
  • decreased CD4-positive NK T cell number / IMPC
  • decreased memory-marker CD4-positive NK T cell number / IMPC
  • decreased Ly6C-positive mature NK cell number / IMPC
  • increased Ly6C-positive NK T cell number / IMPC
  • decreased early germinal center B cell number / IMPC
  • decreased T-helper cell number / IMPC
  • decreased effector memory T-helper cell number / IMPC
  • increased effector memory T-helper cell number / IMPC
  • decreased KLRG1-positive T-helper cell number / IMPC
  • increased KLRG1-positive T-helper cell number / IMPC

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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