B6.129-Criptotm1Eda/Cnrm
| Status | Available to order |
| EMMA ID | EM:12502 |
| International strain name | B6.129-Criptotm1Eda/Cnrm |
| Alternative name | Cripto |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Criptotm1Eda, |
| Gene/Transgene symbol | Cripto |
Information from provider
| Provider | Giovanna Liguori |
| Provider affiliation | Biomedicine, IGB, CNR |
| Genetic information | Mice carrying a null Cripto allele. The gene was targeted by homologous recombination (Xu et al., 1999) |
| Phenotypic information | Homozygous:No homozygous Cripto -/- mice were born, indicating that Cripto is necessary for embryonic development. Between 9.5 and 10.5 d.p.c. Cripto null embryos consisted primarily of extraembryonic tissue including yolk sacs and the developing placenta, while the embryonic region had degenerated. Embryos initiated gastrulation and some produced mesoderm up to 7.5 d.p.c. Increasingly aberrant morphogenesis gave rise to disordered neuroepithelium that failed to produce a recognizable neural tube, or head-fold (Xu et al., 1999). However, despite the severe phenotype, all the telencephalic, diencephalic, mesencephalic, and isthmic territories investigated are specified in the Cripto -/- embryos, with the exception of the anterior neural ridge. Moreover, neural markers that in wild-type embryos define the more rostral regions, are positioned more distally in Cripto -/- mutants, while markers expressed in the more posterior regions of wild-type embryos are located more proximally in the mutants. A complex neural regionalization of anterior character is detectable inside the Cripto embryos, despite the absence of the node and most of the mesoderm (Liguori et al., 2003). Characterization of the functional properties of the neuroectoderm in mouse Cripto -/- embryos shows that they can still form functional neural stem cells but fail to form functional secondary organizers, probably due to reduced expression of the corresponding signalling molecules (Liguori et al., 2009).Heterozygous:Cripto heterozygotes are healthy and fertile (Xu et al., 1999; Liguori et al., 2003). After chronic treatment with the colonotropic carcinogen azoxymethane (AOM), they develop more numerous and larger colon tumors than wild-type mice, some of them being adenocarcinomas (Giorgio et al., 2014). |
| Breeding history | The mice have been backcrossed to C57BL/6 for more than 20 times. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Alobar holoprosencephaly / Orphanet_93925
- Midline interhemispheric variant of holoprosencephaly / Orphanet_93926
- Microform holoprosencephaly / Orphanet_280200
- Septopreoptic holoprosencephaly / Orphanet_280195
- Semilobar holoprosencephaly / Orphanet_220386
- Lobar holoprosencephaly / Orphanet_93924
Literature references
- Characterization of the functional properties of the neuroectoderm in mouse Cripto(-/-) embryos showing severe gastrulation defects.;Liguori Giovanna L, Echevarria Diego, Bonilla Sonia, D'Andrea Daniela, Liguoro Annamaria, Persico Maria G, Martinez Salvador, ;2009;The International journal of developmental biology;53;549-57; 19247965
- Abrogation of the Cripto gene in mouse leads to failure of postgastrulation morphogenesis and lack of differentiation of cardiomyocytes.;Xu C, Liguori G, Persico M G, Adamson E D, ;1999;Development (Cambridge, England);126;483-94; 9876177
- Anterior neural plate regionalization in cripto null mutant mouse embryos in the absence of node and primitive streak.;Liguori Giovanna L, Echevarría Diego, Improta Raffaele, Signore Massimo, Adamson Eileen, Martínez Salvador, Persico M Graziella, ;2003;Developmental biology;264;537-49; 14651936