B6.C3H-Mapk10^m1Mhda/Ieg

Status	Available to order
EMMA ID	EM:12505
International strain name	B6.C3H-Mapk10^m1Mhda/Ieg
Alternative name	Mapk10-Gln100Leu
Strain type	Induced Mutant Strains : Chemically-induced
Allele/Transgene symbol	Mapk10^m1Mhda
Gene/Transgene symbol	Mapk10

Information from provider

Provider	Martin Hrabé de Angelis
Provider affiliation	Institute of Experimental Genetics, Helmholtz Center Munich
Additional owner	Prof. Dr. Jochen Graw, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Institute of Developmental Genetics, Neuherberg/Germany
Genetic information	Mapk10, c. 299, A->T, leading to p. Gln100Leu exchange Disclaimer - Special restrictions: 1) Mutant development was phenotype driven. Although for some mutant lines, linkage data and the molecular characterization of the causative mutation has been published, EMMA has not genotyped the strains and can not provide genotyping protocols. 2) Some lines showed low penetrance of the phenotype. 3) Only sperm available. Rederivation service can not be offered. 4) EMMA quality control standards may not apply for these strains. 5) EMMA has not verified the breeding performance and the genetic background of the strains. The genotype/phenotype of the mice is not confirmed by INFRAFRONTIER/EMMA. We strongly recommend that the recipient confirms genotype/phenotype upon receipt.
Phenotypic information	Homozygous: No obvious phenotype Heterozygous: No obvious phenotype
Breeding history	The mutation was selected from an ENU sperm archive (Sabrautzki S, Kaiser G, Przemeck GKH, Gerst F, Lorza-Gil E, Panse M, Sartorius T, Hoene M, Marschall S, Häring H-U, Hrabe de Angelis M, Ullrich S: Point mutation of Ffar1 abrogates fatty acid-dependent insulin secretion, but protects against HFD-induced glucose intolerance. Mol Metab 6, 2017; 1304-1312). The mutation was originally on a C3HeB/FeJ background, but was outcrossed for 7 generations to C57BL/6JG. The Mapk10-mutant line is free of the rd1 mutation (causing retinal degeneration).
References	None available
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6J

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6.C3H-Mapk10m1Mhda/Ieg