129S2.CFW-Em/JG
| Status | Available to order |
| EMMA ID | EM:12508 |
| International strain name | 129S2.CFW-Em/JG |
| Alternative name | 129-Em/JG (EMORY) |
| Strain type | Spontaneous |
| Allele/Transgene symbol | Em, |
| Gene/Transgene symbol | Em |
Information from provider
| Provider | Jochen Graw |
| Provider affiliation | Institute of Developmental Genetics, Helmholtz Center Munich |
| Genetic information | The age-related, dominant cataract mutation EMORY (gene symbol: Em) arose spontaneously in the breeding colony of Dr. Kuck (Kuck JF, Kuwabara T, Kuck KD: The Emory mouse cataract: an animal model for human senile cataract. Curr Eye Res. 1981-1982;1(11):643-9.). The mutant line was imported into the Helmholtz Research Center (Lab of Prof. Jochen Graw) in 2003 from the Jackson Laboratory (Strain 001998, Bar Harbor, Maine, USA). The underlying mutation is not known. In contrast to the original finding, the cataract mutation appears to be recessive. Disclaimer - Special restrictions: 1) Mutant development was phenotype driven. Although for some mutant lines, linkage data and the molecular characterization of the causative mutation has been published, EMMA has not genotyped the strains and can not provide genotyping protocols. 2) Some lines showed low penetrance of the phenotype. 3) Only sperm available. Rederivation service can not be offered. 4) EMMA quality control standards may not apply for these strains. 5) EMMA has not verified the breeding performance and the genetic background of the strains. The genotype/phenotype of the mice is not confirmed by INFRAFRONTIER/EMMA. We strongly recommend that the recipient confirms genotype/phenotype upon receipt. |
| Phenotypic information | Homozygous:Recessive senile cataract, which appears at the age of 6-8 monthsHeterozygous:No obvious phenotype |
| Breeding history | After import into the Helmholtz Center Munich, the mutant line (on the CFW background) was kept by brother x sister mating without refreshing from the original stock. Beginning of 2016, CFW-EMORY mutant mice was outcrossed to 129S2/SvPasCrl. Offspring were mated inter se and phenotyped for senile cataracts using a slit lamp. Mice with cataracts were selected for further outcross to 129S2/SvPasCrl. In March 2019, the 9th generation of this outcross/intercross scheme was obtained. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
Literature references
- The Emory mouse cataract: an animal model for human senile cataract.;Kuck J F, Kuwabara T, Kuck K D, ;1981;Current eye research;1;643-9; 7346236