B6(Cg)-Mmuttm2.1Mrb/Orl
| Status | Available to order |
| EMMA ID | EM:12576 |
| Citation information | RRID:IMSR_EM:12576 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6(Cg)-Mmuttm2.1Mrb/Orl |
| Alternative name | Mut-flox (B6-Mut tm1Bam/Pg) |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Mmuttm2.1Mrb |
| Gene/Transgene symbol | Mmut |
Information from provider
| Provider | Matthias Baumgartner |
| Provider affiliation | Metabolism, University Children |
| Genetic information | Exon 3 of the gene methylmalonyl-CoA mutase (Mmut) was targeted to be flanked by loxP sites. For the generation of targeted ES cell clones, C57BL/6 derived ES cells were electroporated with a linearized EUCOMM vector (EUCOMM vector ID 47381). In the targeting vector, exon 3 is flanked by loxP sites; additionally an FRT-flanked targeting cassette was inserted into intron 2. This cassette included a neomycin resistance gene and a lacZ reporter, which consisted of splice acceptor (En2 SA), IRES and beta-gal. Homologous recombination in ES cells was verified by PCR screening, restriction enzyme digest and Southern Blot. A positive ES cell clone was injected into the blastocysts of C57BL/6, resulting in chimeric mice which were set up for breeding with flp-deleter mice. The offspring was PCR screened for i) the presence of the remaining FRT-site in the targeted allele to identify germ line transmitters; ii) the absence of the neomycin cassette; iii) the distal loxP site; iv) and the flp transgene. |
| Phenotypic information | Homozygous:There is no phenotype without cross-breeding to cre recombinase-expressing mice. The phenotype of the resulting matings depends on the cre recombinase promoter.Heterozygous:No phenotype. |
| Breeding history | Mice were kept as homozygous Mmut flox/flox, by breeding Mmut flox/flox mice with other Mmut flox/flox mice from the same line. More than 20 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | homozygous C57BL/6 males |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Vitamin B12-unresponsive methylmalonic acidemia type mut0 / Orphanet_289916
- Vitamin B12-unresponsive methylmalonic acidemia type mut- / Orphanet_79312
Literature references
- New in vitro model derived from brain-specific Mut-/- mice confirms cerebral ammonium accumulation in methylmalonic aciduria.;Remacle Noémie, Forny Patrick, Cudré-Cung Hong-Phuc, Gonzalez-Melo Mary, do Vale-Pereira Sónia, Henry Hugues, Teav Tony, Gallart-Ayala Hector, Braissant Olivier, Baumgartner Matthias, Ballhausen Diana, ;2018;Molecular genetics and metabolism;124;266-277; 29934063