C57BL/6N-Cep85lem1Ciphe/Orl

Status

Available to order

EMMA IDEM:12612
Citation informationRRID:IMSR_EM:12612 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

International strain nameC57BL/6N-Cep85lem1Ciphe/Orl
Alternative nameCep85l em1Ciphe
Strain typeEndonuclease-mediated
Allele/Transgene symbolCep85lem1Ciphe
Gene/Transgene symbolCep85l

Information from provider

ProviderMarie Malissen
Provider affiliationCIPHE
Genetic informationMutation: complete deletion of exon 1. Mutation in Cep85l gene using CRISPR/Cas technology in C57BL/6N eggs. The obtained mutation corresponds to a 174 bp deletion encompassing exon 1. The selected mutation was verified by genomic sequencing.
Phenotypic informationHomozygous:
NA

Heterozygous:
NA
Breeding historyBackcrossed 3 times to C57BL/6N mice.
References
  • Quantitative interactomics in primary T cells unveils TCR signal diversification extent and dynamics.;Voisinne Guillaume, Kersse Kristof, Chaoui Karima, Lu Liaoxun, Chaix Julie, Zhang Lichen, Goncalves Menoita Marisa, Girard Laura, Ounoughene Youcef, Wang Hui, Burlet-Schiltz Odile, Luche Hervé, Fiore Frédéric, Malissen Marie, Gonzalez de Peredo Anne, Liang Yinming, Roncagalli Romain, Malissen Bernard, ;2019;Nature immunology;20;1530-1541; 31591574
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreCNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France

Disease and phenotype information

IMPC phenotypes (gene matching)
  • abnormal auditory brainstem response / IMPC
  • decreased prepulse inhibition / IMPC
  • decreased startle reflex / IMPC
  • abnormal lymph node morphology / IMPC
  • abnormal startle reflex / IMPC
  • enlarged kidney / IMPC
  • abnormal skin morphology / IMPC
  • abnormal kidney morphology / IMPC
  • abnormal behavior / IMPC
  • enlarged lymph nodes / IMPC
  • decreased thigmotaxis / IMPC
  • decreased anxiety-related response / IMPC

Literature references

  • Quantitative interactomics in primary T cells unveils TCR signal diversification extent and dynamics.;Voisinne Guillaume, Kersse Kristof, Chaoui Karima, Lu Liaoxun, Chaix Julie, Zhang Lichen, Goncalves Menoita Marisa, Girard Laura, Ounoughene Youcef, Wang Hui, Burlet-Schiltz Odile, Luche Hervé, Fiore Frédéric, Malissen Marie, Gonzalez de Peredo Anne, Liang Yinming, Roncagalli Romain, Malissen Bernard, ;2019;Nature immunology;20;1530-1541; 31591574

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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