C57BL/6N-Ern1em1Tac/Orl
| Status | Available to order |
| EMMA ID | EM:12658 |
| Citation information | RRID:IMSR_EM:12658 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6N-Ern1em1Tac/Orl |
| Alternative name | ERN1 R594C/WT |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Ern1em1Tac |
| Gene/Transgene symbol | Ern1 |
Information from provider
| Provider | Pauline Soulas-Sprauel |
| Provider affiliation | CRBS (Centre de Recherche en Biomédecine), Inserm UMR 1109 (Immuno-Rhumatologie Moléculaire, IRM) |
| Genetic information | The Ern1 gene has been mutated per CRISPR/Cas9 technology by Taconic (single point mutation C1780T leading to R594C substitution). This heterozygous mutation has been detected in a family with several members affected by autoimmunity and lupus in the proband (Agence nationale de la recherche-ANR project LUMUGENE). Founders initially generated on C57BL/6NTac background; backcrossed 5 times to C57BL/6N (The Jackson Lab.). |
| Phenotypic information | Homozygous:Not known yetHeterozygous:Heterozygous mice: - Decrease of T cell compartment in spleen and lymph nodes from old mice (9 months and older), particularly naïve T cells (CD44lowCD62L+) - Increased level of serum IgM (starting at 9 months old) - Autoantibodies detected by antigen microarray in serum from old mice (9 to 12 months old) |
| Breeding history | Backcrossed to C57BL/6N (The Jackson Lab.), currently 5th generation. |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |