B6NCrl;B6N-A^tm1Brd Tdgf1^{tm1a(EUCOMM)Hmgu}/Ieg

Status	Available to order
EMMA ID	EM:12813
Citation information	RRID:IMSR_EM:12813 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6NCrl;B6N-A^tm1Brd Tdgf1^{tm1a(EUCOMM)Hmgu}/Ieg
Alternative name	HEPD0737_8_E09
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Tdgf1^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Cripto
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0737_8_E09. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6N males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Alobar holoprosencephaly / Orphanet_93925
Midline interhemispheric variant of holoprosencephaly / Orphanet_93926
Microform holoprosencephaly / Orphanet_280200
Septopreoptic holoprosencephaly / Orphanet_280195
Semilobar holoprosencephaly / Orphanet_220386
Lobar holoprosencephaly / Orphanet_93924

IMPC phenotypes (gene matching)

preweaning lethality, complete penetrance / IMPC

MGI phenotypes (gene matching)

abnormal heart morphology / MGI
abnormal heart development / MGI
absent notochord / MGI
abnormal germ layer development / MGI
abnormal mesoderm development / MGI
absent mesoderm / MGI
abnormal endoderm development / MGI
failure of primitive streak formation / MGI
abnormal gastrulation / MGI
abnormal embryo turning / MGI
absent vitelline blood vessels / MGI
embryonic growth arrest / MGI
premature death / MGI
abnormal developmental patterning / MGI
abnormal embryonic tissue morphology / MGI
abnormal extraembryonic tissue morphology / MGI
no abnormal phenotype detected / MGI
abnormal primitive streak formation / MGI
abnormal chorion morphology / MGI
abnormal cell adhesion / MGI
abnormal embryonic neuroepithelium morphology / MGI
absent prechordal plate / MGI
abnormal amnion morphology / MGI
holoprosencephaly / MGI
cyclopia / MGI
abnormal rostral-caudal axis patterning / MGI
embryo phenotype / MGI
absent somites / MGI
abnormal mesendoderm development / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
abnormal visceral endoderm morphology / MGI
abnormal visceral yolk sac blood island morphology / MGI
abnormal neural fold morphology / MGI
decreased fibroblast proliferation / MGI
fused somites / MGI
decreased midbrain size / MGI
decreased forebrain size / MGI
rostral body truncation / MGI
short rostral-caudal axis / MGI
absent anterior definitive endoderm / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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