B6.Cg-Trp73tm1.1Gml/H
| Status | Available to order |
| EMMA ID | EM:13068 |
| International strain name | B6.Cg-Trp73tm1.1Gml/H |
| Alternative name | Trp73D13/D13 (Trp73delta13) |
| Strain type | Targeted Mutant Strains : Other targeted |
| Allele/Transgene symbol | targeted mutation 1.1, Gerry Melino |
| Gene/Transgene symbol | Trp73 |
Information from provider
| Provider | Gerry Melino |
| Provider affiliation | MRC Toxicology Unit |
| Genetic information | Trp73D13/D13 mice were generated using the cre-loxP system. First, floxed (Trp73fl/fl) mice (EMMA strain EM:13067) were obtained by introducing a vector containing loxP sites flanking exon 13 of the Trp73 gene, which was replaced by a neoR cassette to enable selection. Immediately upstream and downstream of these sites, long terminal repeats (LTRs) facilitated incorporation of the vector into ES cells by homologous recombination. Mice where then crossed with Flp recombinase expressing animals to remove the NeoR cassette. Floxed mice were subsequently crossed with mice ubiquitously expressing cre recombinase under the human cytomegalovirus promoter (CMV-cre) to delete exon 13 in all tissues. |
| Phenotypic information | Homozygous:Trp73D13/D13 mice are significantly smaller than control mice beginning at P7, and 40% of them die within the first 3 weeks of life. The hippocampal architecture of Trp73D13/D13 mice is completely misshapen. Hippocampal neurons are able to form CA and DG cell layers, but these regions are morphologically misassembled and displayed a severely altered architecture. The observed hippocampal dysgenesis strongly impairs memory and learning, and it determines a reduction of synaptic transmission. Trp73D13/D13 also show an impaired adult neurogenesis process. Furthermore, a massive loss of Cajal-Retzius cells is observed in the Trp73D13/D13 mice.Heterozygous:No abnormal phenotype is observed in heterozygous mice (compared to wild-type littermates). |
| Breeding history | Backcrossed more than 10 generations. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- The C terminus of p73 is essential for hippocampal development.;Amelio Ivano, Panatta Emanuele, Niklison-Chirou Maria Victoria, Steinert Joern R, Agostini Massimiliano, Morone Nobuhiro, Knight Richard A, Melino Gerry, ;2020;Proceedings of the National Academy of Sciences of the United States of America;117;15694-15701; 32571922
- P73 C-terminus is dispensable for multiciliogenesis.;Buckley Niall, Panatta Emanuele, Morone Nobuhiro, Noguchi Masafumi, Scorrano Luca, Knight Richard A, Amelio Ivano, Melino Gerry, ;2020;Cell cycle (Georgetown, Tex.);19;1833-1845; 32584647