B6;CBA-Tln1^em1Flmg/Flmg

Status	Available to order
EMMA ID	EM:13074
Citation information	RRID:IMSR_EM:13074 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;CBA-Tln1^em1Flmg/Flmg
Alternative name	Talin1 C-mutant
Strain type	Endonuclease-mediated
Allele/Transgene symbol	Tln1^em1Flmg
Gene/Transgene symbol	Tln1

Information from provider

Provider	Transgenics Facility B.S.R.C.
Provider affiliation	B.S.R.C. "Alexander Fleming"
Genetic information	Mice with a deletion of exons 55-57 of talin 1 gene were generated using CRISPR/Cas9 technology. Two guide RNAs were designed to target sequences outside of the selected area. A ssODN template consisting of two 60 bp fragments at each of the outer sides of the Cas9 cutting edges was used producing a deletion of 1033 bp. The CRISPR components were introduced into zygotes via the embryo electroporation method.
Phenotypic information	Homozygous: Embryonic lethal Heterozygous: No observable phenotype
Breeding history	The colony has been maintained by backcrossing talin 1 C mutant heterozygous mice to C57BL/6J mice for six generations; talin 1 C mutant homozygotes are embryonic lethal.
References	None available
Homozygous fertile	no
Homozygous viable	no
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	B.S.R.C. Alexander Fleming, Vari, Greece
Animals used for archiving	heterozygous CBA x C57BL/6 males, wild-type C57BL/6J females
Stage of embryos	Morula

Disease and phenotype information

MGI phenotypes (gene matching)

abnormal embryo development / MGI
abnormal ectoderm development / MGI
abnormal mesoderm development / MGI
decreased embryo size / MGI
embryonic growth arrest / MGI
abnormal embryonic tissue morphology / MGI
abnormal extraembryonic tissue morphology / MGI
abnormal gastrulation movements / MGI
disorganized extraembryonic tissue / MGI
abnormal trophoblast layer morphology / MGI
abnormal extraembryonic mesoderm development / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality between somite formation and embryo turning, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
abnormal proamniotic cavity morphology / MGI
abnormal amniotic cavity morphology / MGI
absent ectoplacental cavity / MGI
small proamniotic cavity / MGI
absent extraembryonic coelom / MGI
small amniotic cavity / MGI
embryonic-extraembryonic boundary constriction / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

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B6;CBA-Tln1em1Flmg/Flmg