C57BL/6-Slc20a2tm1c(EUCOMM)Wtsi/Orl
| Status | Available to order |
| EMMA ID | EM:13085 |
| International strain name | C57BL/6-Slc20a2tm1c(EUCOMM)Wtsi/Orl |
| Alternative name | Slc20a2 |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Slc20a2tm1c(EUCOMM)Wtsi, |
| Gene/Transgene symbol | Slc20a2 |
Information from provider
| Provider | Nati Hernando |
| Provider affiliation | Physiology, University Zurich |
| Additional owner | Dr. Carsten Wagner, Institute of Physiology, University Zurich, CH |
| Genetic information | Embryonic stem (ES) cells transfected with a Slc20a2 targeting vector were purchased from the International Knockout Mouse Consortium (IKMC)/ European Conditional Mouse Mutagenesis Program (EUCOMM). In these ES cells, the exon 4 of the sodium/phosphate cotransporter Slc20a2/Pit-2 was flanked by loxP sites. Upstream of the 5’ loxP site, the vector contained a cassette for production of beta-galactosidase, followed by a neomycin phosphotransferase gene cassette. ES cells were sent to the Institute of Labortierkunde (LTK) of the University of Zurich for morula aggregation and implantation into foster mothers. Upon breeding to homozygosity, floxed/floxed mice were crossed with flp recombinase full deleters (64FlpB6) for removal of the beta-gal/neo cassettes. |
| Phenotypic information | Homozygous:An intestinal-specific knockout was generated by breeding floxed Slc20a2 tm1c mice with villin-cre mice, in which the expression of the cre recombinase is under the control of the villin promoter. No differences in phosphate homeostasis were found between wild-types and homozygous littermates (Pastor-Arroyo et al., 2020, PubMed ID: 32564464).Heterozygous:Heterozygous mice were not analysed, but they are not expected to have any phenotype since homozygous mice did not show any. |
| Breeding history | Mice breed without problems. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
Literature references
- Intestinal epithelial ablation of Pit-2/Slc20a2 in mice leads to sustained elevation of vitamin D3 upon dietary restriction of phosphate.;Pastor-Arroyo Eva M, Knöpfel Thomas, Imenez Silva Pedro H, Schnitzbauer Udo, Poncet Nadège, Biber Jürg, Wagner Carsten A, Hernando Nati, ;2020;Acta physiologica (Oxford, England);230;e13526; 32564464