| Status | Available to order |
| EMMA ID | EM:13092 |
| Citation information | RRID:IMSR_EM:13092 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6J-Gzmbem1Bros/Orl |
| Alternative name | GzmB-mTFP KI |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Gzmbem1Bros |
| Gene/Transgene symbol | Gzmb |
| Provider | Jens Rettig |
| Provider affiliation | Physiological Institute/CIPMM, University of Saarland |
| Genetic information | GzmB-mTFP KI mice were generated by CRISPR/Cas9 technology with an HDR fragment designed to replace the endogenous Stop codon of the Gzmb gene by sequences encoding a GGSGGSGGS-linker, mTFP (teal fluorescent protein reporter) and a Stop codon. |
| Phenotypic information | Homozygous:Mice are healthy and fertile. No obvious phenotype could be detected.Heterozygous:Mice are healthy and fertile. No obvious phenotype could be detected. |
| Breeding history | Offspring were genotyped for the desired mutation and further bred with C57BL/6J. The genetically background is fixed with CRISPR/Cas9. No backcrossing is necessary. Since the creation of the strain we bred animals up to F8. Animals can be bred homogeneously. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | homozygous C57BL/6J males |
