- increased inflammatory response / MGI
- male infertility / MGI
- abnormal seminiferous tubule morphology / MGI
- abnormal epididymis morphology / MGI
- abnormal keratinocyte differentiation / MGI
- epididymal inflammation / MGI
- increased incidence of tumors by chemical induction / MGI
- abnormal epididymis epithelium morphology / MGI
- decreased tumor latency / MGI
- altered tumor pathology / MGI
- testis degeneration / MGI
- epididymis hypertrophy / MGI
- spermatocele / MGI
- epididymis fibrosis / MGI
- increased epididymal cell proliferation / MGI
- increased epididymal epithelium cell proliferation / MGI
B6.Cg-Gt(ROSA)26Sortm1.1(Loxl2)Acan/Cnbc
Status | Available to order |
EMMA ID | EM:13122 |
International strain name | B6.Cg-Gt(ROSA)26Sortm1.1(Loxl2)Acan/Cnbc |
Alternative name | ODBbl6 |
Strain type | Targeted Mutant Strains : Knock-in |
Allele/Transgene symbol | Gt(ROSA)26Sortm1.1(Loxl2)Acan, |
Gene/Transgene symbol | Gt(ROSA)26Sor |
Information from provider
Provider | Francisco Portillo |
Provider affiliation | Biochemistry, Universidad Autónoma Madrid |
Additional owner | Amparo Cano and Alberto Martin, Universidad Autónoma de Madrid, Madrid, Spain |
Genetic information | Loxl2 constitutive knock-in. |
Phenotypic information | Homozygous:Two Scapers homozygous mutant males were fertile in the C57BL/6J genetic background. This contrasts with the sterile phenotype observed on the original ODB strain in a mixed genetic background due to epididymal and testicular dysfunction (see EMMA strain EM:12917). Initial crosses of mice in the C57BL/6J background show almost complete perinatal lethality of homozygous mutant mice. Heterozygous:Normal and fertile |
Breeding history | Loxl2 cDNA followed by a GFP reporter gene and an upstream PGK-neo cassette flanked by two loxP sites was introduced by homologous recombination in the endogenous Gt(ROSA)26Sor locus. ES clones positive for the mutation were microinjected/aggregated into receptive blastocysts/morulae and chimeras obtained. These animals were then crossed with cre recombinase-expressing mice to check for germline transmision and, at the same time, obtain animals constitutively expressing extra amounts of Loxl2. The resulting heterozygous animals were intercrossed and animals harbouring two Loxl2 overexpression alleles were obtained. Homozygous females in a mixed genetic background were intercrossed with C57BL/6J males for 12 generations to obtain animals harbouring two Loxl2 overexpression alleles in a pure C57BL/6J background. |
References |
|
Homozygous fertile | yes |
Homozygous viable | no |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
Animals used for archiving | heterozygous C57BL/6J |
Disease and phenotype information
MGI phenotypes (allele matching)
Literature references
- Lysyl oxidase-like 2 represses Notch1 expression in the skin to promote squamous cell carcinoma progression.;Martin Alberto, Salvador Fernando, Moreno-Bueno Gema, Floristán Alfredo, Ruiz-Herguido Cristina, Cuevas Eva P, Morales Saleta, Santos Vanesa, Csiszar Katalin, Dubus Pierre, Haigh Jody J, Bigas Anna, Portillo Francisco, Cano Amparo, ;2015;The EMBO journal;34;1090-109; 25759215
Information on how we integrate external resources can be found here
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).