B6.Cg-Gt(ROSA)26Sortm1.1(Loxl2)Acan/Cnbc

Status

Available to order

EMMA IDEM:13122
International strain nameB6.Cg-Gt(ROSA)26Sortm1.1(Loxl2)Acan/Cnbc
Alternative nameODBbl6
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolGt(ROSA)26Sortm1.1(Loxl2)Acan,
Gene/Transgene symbolGt(ROSA)26Sor

Information from provider

ProviderFrancisco Portillo
Provider affiliationBiochemistry, Universidad Autónoma Madrid
Additional ownerAmparo Cano and Alberto Martin, Universidad Autónoma de Madrid, Madrid, Spain
Genetic informationLoxl2 constitutive knock-in.
Phenotypic informationHomozygous:
Two Scapers homozygous mutant males were fertile in the C57BL/6J genetic background. This contrasts with the sterile phenotype observed on the original ODB strain in a mixed genetic background due to epididymal and testicular dysfunction (see EMMA strain EM:12917). Initial crosses of mice in the C57BL/6J background show almost complete perinatal lethality of homozygous mutant mice.

Heterozygous:
Normal and fertile
Breeding historyLoxl2 cDNA followed by a GFP reporter gene and an upstream PGK-neo cassette flanked by two loxP sites was introduced by homologous recombination in the endogenous Gt(ROSA)26Sor locus. ES clones positive for the mutation were microinjected/aggregated into receptive blastocysts/morulae and chimeras obtained. These animals were then crossed with cre recombinase-expressing mice to check for germline transmision and, at the same time, obtain animals constitutively expressing extra amounts of Loxl2. The resulting heterozygous animals were intercrossed and animals harbouring two Loxl2 overexpression alleles were obtained. Homozygous females in a mixed genetic background were intercrossed with C57BL/6J males for 12 generations to obtain animals harbouring two Loxl2 overexpression alleles in a pure C57BL/6J background.
References
  • Lysyl oxidase-like 2 represses Notch1 expression in the skin to promote squamous cell carcinoma progression.;Martin Alberto, Salvador Fernando, Moreno-Bueno Gema, Floristán Alfredo, Ruiz-Herguido Cristina, Cuevas Eva P, Morales Saleta, Santos Vanesa, Csiszar Katalin, Dubus Pierre, Haigh Jody J, Bigas Anna, Portillo Francisco, Cano Amparo, ;2015;The EMBO journal;34;1090-109; 25759215
Homozygous fertileyes
Homozygous viableno
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous C57BL/6J

Disease and phenotype information

MGI phenotypes (allele matching)
  • increased inflammatory response / MGI
  • male infertility / MGI
  • abnormal seminiferous tubule morphology / MGI
  • abnormal epididymis morphology / MGI
  • abnormal keratinocyte differentiation / MGI
  • epididymal inflammation / MGI
  • increased incidence of tumors by chemical induction / MGI
  • abnormal epididymis epithelium morphology / MGI
  • decreased tumor latency / MGI
  • altered tumor pathology / MGI
  • testis degeneration / MGI
  • epididymis hypertrophy / MGI
  • spermatocele / MGI
  • epididymis fibrosis / MGI
  • increased epididymal cell proliferation / MGI
  • increased epididymal epithelium cell proliferation / MGI

Literature references

  • Lysyl oxidase-like 2 represses Notch1 expression in the skin to promote squamous cell carcinoma progression.;Martin Alberto, Salvador Fernando, Moreno-Bueno Gema, Floristán Alfredo, Ruiz-Herguido Cristina, Cuevas Eva P, Morales Saleta, Santos Vanesa, Csiszar Katalin, Dubus Pierre, Haigh Jody J, Bigas Anna, Portillo Francisco, Cano Amparo, ;2015;The EMBO journal;34;1090-109; 25759215

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).