IMPC phenotypes (gene matching)
- preweaning lethality, incomplete penetrance / IMPC
B6.Cg-Loxl2tm1.2Acan/Cnbc
| Status | Available to order |
| EMMA ID | EM:13123 |
| Citation information | RRID:IMSR_EM:13123 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Loxl2tm1.2Acan/Cnbc |
| Alternative name | CFBbl6 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Loxl2tm1.2Acan |
| Gene/Transgene symbol | Loxl2 |
Information from provider
| Provider | Francisco Portillo |
| Provider affiliation | Biochemistry, Universidad Autónoma de Madrid |
| Additional owner | Amparo Cano and Alberto Martin, Universidad Autónoma de Madrid, Madrid, Spain |
| Genetic information | Loxl2 constitutive knock-out. |
| Phenotypic information | Homozygous:Lethality of homozygous mutant mice observed in 100% crosses of heterozygous mice analysed so far. This contrasts with the partial perinatal lethality observed in homozygous mutant mice in a mixed genetic background (see EMMA strains EM:12882 and EM:12916).Heterozygous:Apparently normal |
| Breeding history | Conditional knock-out mutation of Loxl2 based on cre/loxP system was introduced by homologous recombination in the endogenous locus of Loxl2. ES clones positive for the mutation were microinjected/aggregated into receptive blastocysts/morulae and chimeras obtained. These animals were crossed with cre recombinase-expressing mice to check germline transmission and, at the same time, to obtain animals harbouring a constitutive knock-out allele. The resultant heterozygous animals were intercrossed and knock-out animals obtained. Homozygous females in a mixed genetic background were intercrossed with C57BL/6J males for 12 generations to obtain animals harbouring a constitutive knock-out allele in a pure C57BL/6J background. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6J males |
Disease and phenotype information
Literature references
- Lysyl oxidase-like 2 represses Notch1 expression in the skin to promote squamous cell carcinoma progression.;Martin Alberto, Salvador Fernando, Moreno-Bueno Gema, Floristán Alfredo, Ruiz-Herguido Cristina, Cuevas Eva P, Morales Saleta, Santos Vanesa, Csiszar Katalin, Dubus Pierre, Haigh Jody J, Bigas Anna, Portillo Francisco, Cano Amparo, ;2015;The EMBO journal;34;1090-109; 25759215