B6.129S1-Adgre1tm1Stst/H
| Status | Available to order |
| EMMA ID | EM:13139 |
| International strain name | B6.129S1-Adgre1tm1Stst/H |
| Alternative name | Emr1 knockout |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Adgre1tm1Stst, |
| Gene/Transgene symbol | Adgre1 |
Information from provider
| Provider | Hsi-Hsien Lin |
| Provider affiliation | Department of Microbiology and Immunology, College of Medicine, Chang Gung University |
| Additional owner | Prof. Siamon Gordon, Department of Pathology, University of Oxford, United Kingdom |
| Genetic information | The murine Adgre1 (F4/80 or Emr1) gene is located on the distal region of Chromosome 17. An F4/80-targeting construct was prepared by replacing an 1.0-kb HindIII–XbaI fragment containing the coding sequence of the first exon and the 5' end of the first intron with a promoterless-galactosidase/pGK-Neo cassette. The homologous regions in both arms of the targeting construct are a 1.8-kb fragment 5' to the first exon and a 2.8-kb fragment containing the exon 2 and the 3' end of intron 1. |
| Phenotypic information | Homozygous:The mouse macrophage-restricted ADGRE1 (F4/80) protein is not required for the development and distribution of tissue macrophages but is involved in the generation of antigen-specific efferent regulatory T (Treg) cells that suppress antigen-specific immunity. In the in vivo anterior chamber (a.c.)-associated immune deviation (ACAID) model of peripheral tolerance, a.c. inoculation of antigen into F4/80-/- mice was unable to induce efferent Treg cells and suppress delayed-type hypersensitivity (DTH) responses. Moreover, the use of anti-F4/80 mAb and F4/80-/- APCs in an in vitro ACAID model showed that all antigen presenting cells (APCs) in the culture must be able to express the F4/80 protein if efferent Treg cells were to be generated. In a low-dose oral tolerance model, wild-type but not F4/80-/- mice generated an efferent CD8+ Treg cell population that suppressed an antigen-specific DTH response. Peripheral tolerance was restored in F4/80-/- mice by adoptive transfer of F4/80+ APCs in both peripheral tolerance models, indicating a central role for the F4/80 molecule in the generation of efferent CD8+ Treg cells. In conclusion, the macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.Heterozygous:Same as wild-type. |
| Breeding history | Adgre1 (Emr1) knock-out mice were backcrossed to a C57BL/6J line for 10 generations and the established homozygous line has been maintained as brother x sister mate within the Univ. of Oxford facility. They breed just as good as the background strain with average litter sizes of 5/7 pups and the health status has remained consistent for many years. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.;Lin Hsi-Hsien, Faunce Douglas E, Stacey Martin, Terajewicz Ania, Nakamura Takahiko, Zhang-Hoover Jie, Kerley Marilyn, Mucenski Michael L, Gordon Siamon, Stein-Streilein Joan, ;2005;The Journal of experimental medicine;201;1615-25; 15883173