C57BL/6-Ago2^em1Jghy/H

Status	Available to order
EMMA ID	EM:13149
Citation information	RRID:IMSR_EM:13149 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6-Ago2^em1Jghy/H
Alternative name	Ago2 S388A
Strain type	Endonuclease-mediated
Allele/Transgene symbol	Ago2^em1Jghy
Gene/Transgene symbol	Ago2

Information from provider

Provider	Jonathan Hanley
Provider affiliation	Biochemistry, University of Bristol
Genetic information	Point mutation in the argonaute 2 (Ago2) gene made by CRISPR/Cas9 gene editing: AGT (Ser) to GCT (Ala), Ser 388 mutated to Ala.
Phenotypic information	Homozygous: Unknown Heterozygous: Grossly normal, but detailed characterisation not yet carried out, since phenotyping still ongoing. Please enquire for more information.
Breeding history	F0 animal was bred onto C57BL/6J and the resulting offspring were then bred again to C57BL/6J to generate the F2 animals.
References	None available
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Non-specific syndromic intellectual disability / Orphanet_528084

MGI phenotypes (gene matching)

enlarged heart / MGI
open neural tube / MGI
wavy neural tube / MGI
abnormal placenta morphology / MGI
placental labyrinth hypoplasia / MGI
abnormal visceral yolk sac morphology / MGI
embryonic growth arrest / MGI
pericardial edema / MGI
abnormal embryonic tissue morphology / MGI
abnormal proerythroblast morphology / MGI
decreased erythrocyte cell number / MGI
no phenotypic analysis / MGI
abnormal forebrain development / MGI
pallor / MGI
embryonic growth retardation / MGI
small placenta / MGI
cellular phenotype / MGI
neonatal lethality, complete penetrance / MGI
prenatal lethality, complete penetrance / MGI
embryonic lethality between implantation and somite formation, complete penetrance / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Example health report
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Material Transfer Agreement (MTA)
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Legally binding conditions for the transfer

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C57BL/6-Ago2em1Jghy/H