B6JOlaHsd.Cg-Tg(FUS)2Kt/H

Status	Available to order
EMMA ID	EM:13155
Citation information	RRID:IMSR_EM:13155 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6JOlaHsd.Cg-Tg(FUS)2Kt/H
Alternative name	Tg (WT Fus) 2 Kt
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(FUS)2Kt
Gene/Transgene symbol	Tg(FUS)2Kt

Information from provider

Provider	Kevin Talbot
Provider affiliation	Nuffield Department of Clinical Neuroscience, University of Oxford
Genetic information	Two BAC constructs containing genomic wild-type and P525L FUS (fused in sarcoma) were generated by subcloning the human genomic FUS locus from a 225 kb BAC, RP11-388M20 (BACPAC resource centre, Children’s Hospital Oakland Research Institute), to a pCYPAC2 backbone. The pCYPAC2 backbone was then swapped for pBACe3.6, and loxP sites and a mCherry tag were introduced. The exon 15 mutation (c. 1574C-T) was inserted separately by site-directed mutagenesis.
Phenotypic information	Homozygous: Not known Heterozygous: This particular strain (BAC-FUS-WT mice, EMMA strain ID EM:13155) has no known phenotypic changes relative to non-transgenic controls while BAC-FUS-P525L mice (EMMA strain ID EM:13158) have an early onset hyperactive phenotype on open field testing. FUS-P525L-mCherry is mislocalized to the cytoplasm in primary motor neurons in vitro and in spinal cord tissue.
Breeding history	Founder mice (C57BL/6 x CBA)F1 were backcrossed to ~98% C57BL/6JOlaHsd (Harlan) and maintained on this background.
References	None available
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Amyotrophic lateral sclerosis / Orphanet_803
Juvenile amyotrophic lateral sclerosis / Orphanet_300605

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

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Practical information

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