C57BL/6NTac-Map3k1tm1a(KOMP)Wtsi/WtsiFlmg

Status

Available to order

EMMA IDEM:13176
Citation informationRRID:IMSR_EM:13176 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6NTac-Map3k1tm1a(KOMP)Wtsi/WtsiFlmg
Alternative nameEPD0287_3_A11
Strain typeTargeted Mutant Strains
Allele/Transgene symbolMap3k1tm1a(KOMP)Wtsi
Gene/Transgene symbolMap3k1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from KOMP ES clone EPD0287_3_A11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreB.S.R.C. Alexander Fleming, Vari, Greece

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • abnormal retina morphology / IMPC
  • increased circulating free fatty acids level / IMPC
  • increased lean body mass / IMPC
  • increased red blood cell distribution width / IMPC
  • decreased circulating fructosamine level / IMPC
  • increased bone mineral content / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
  • decreased fasting circulating glucose level / IMPC
IMPC phenotypes (gene matching)
  • increased red blood cell distribution width / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
  • abnormal retina morphology / IMPC
  • abnormal startle reflex / IMPC
  • increased lean body mass / IMPC
  • decreased fasting circulating glucose level / IMPC
  • increased bone mineral content / IMPC
  • increased circulating free fatty acids level / IMPC
  • decreased circulating fructosamine level / IMPC
MGI phenotypes (gene matching)
  • organ of Corti degeneration / MGI
  • microphthalmia / MGI
  • eyelids open at birth / MGI
  • corneal opacity / MGI
  • abnormal eyelid morphology / MGI
  • abnormal pinna reflex / MGI
  • heart inflammation / MGI
  • abnormal eye morphology / MGI
  • abnormal keratinocyte morphology / MGI
  • exophthalmos / MGI
  • early eyelid opening / MGI
  • increased cardiomyocyte apoptosis / MGI
  • abnormal retinal layer morphology / MGI
  • abnormal heart left ventricle morphology / MGI
  • decreased cochlear inner hair cell number / MGI
  • increased cochlear outer hair cell number / MGI
  • cochlear outer hair cell degeneration / MGI
  • increased response of heart to induced stress / MGI
  • abnormal vascular wound healing / MGI
  • abnormal eye physiology / MGI
  • hearing/vestibular/ear phenotype / MGI
  • immune system phenotype / MGI
  • abnormal vascular smooth muscle physiology / MGI
  • decreased ventricle muscle contractility / MGI
  • congestive heart failure / MGI
  • abnormal eyelid fusion / MGI
  • abnormal eyelid development / MGI
  • increased sensitivity to induced morbidity/mortality / MGI
  • impaired fibroblast cell migration / MGI
  • increased or absent threshold for auditory brainstem response / MGI
  • abnormal eyelid margin morphology / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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