B6JOlaHsd.Cg-Tg(FUS*P525L)3Kt/H

Status	Available to order
EMMA ID	EM:13211
Citation information	RRID:IMSR_EM:13211 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6JOlaHsd.Cg-Tg(FUS*P525L)3Kt/H
Alternative name	Tg (P525L Fus) 3 Kt
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(FUS*P525L)3Kt
Gene/Transgene symbol	Tg(FUS*P525L)3Kt

Information from provider

Provider	Kevin Talbot
Provider affiliation	Nuffield Department of Clinical Neuroscience, University of Oxford
Genetic information	Two BAC constructs containing genomic wild-type and P525L FUS (fused in sarcoma) were generated by subcloning the human genomic FUS locus from a 225 kb BAC, RP11-388M20 (BACPAC resource centre, Children’s Hospital Oakland Research Institute), to a pCYPAC2 backbone. The pCYPAC2 backbone was then swapped for pBACe3.6, and loxP sites and an mCherry tag were introduced. The exon 15 mutation (c. 1574C>T) was inserted separately by site-directed mutagenesis.
Phenotypic information	Homozygous: Not known Heterozygous: BAC-FUS-WT mice have no known phenotypic changes relative to non-transgenic controls. BAC-FUS-P525L mice have an early onset hyperactive phenotype on open field testing. FUS-P525L mCherry is mislocalized to the cytoplasm in primary motor neurons in vitro and in spinal cord tissue. These mice are considered 'high' expressers compared to Tg(FUS*P525L)1Kt mice (EMMA strain ID EM:13158).
Breeding history	Founder mice (C57BL/6 x CBA)F1 were backcrossed to ~98% C57BL/6JOlaHsd (Harlan) and maintained on this background.
References	None available
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

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Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
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