B6JOlaHsd.Cg-Tg(FUS*P525L)3Kt/H
Status | Available to order |
EMMA ID | EM:13211 |
International strain name | B6JOlaHsd.Cg-Tg(FUS*P525L)3Kt/H |
Alternative name | Tg (P525L Fus) 3 Kt |
Strain type | Transgenic Strains |
Allele/Transgene symbol | Tg(FUS*P525L)3Kt, |
Gene/Transgene symbol | Tg(FUS*P525L)3Kt |
Information from provider
Provider | Kevin Talbot |
Provider affiliation | Nuffield Department of Clinical Neuroscience, University of Oxford |
Genetic information | Two BAC constructs containing genomic wild-type and P525L FUS (fused in sarcoma) were generated by subcloning the human genomic FUS locus from a 225 kb BAC, RP11-388M20 (BACPAC resource centre, Children’s Hospital Oakland Research Institute), to a pCYPAC2 backbone. The pCYPAC2 backbone was then swapped for pBACe3.6, and loxP sites and an mCherry tag were introduced. The exon 15 mutation (c. 1574C>T) was inserted separately by site-directed mutagenesis. |
Phenotypic information | Homozygous:Not knownHeterozygous:BAC-FUS-WT mice have no known phenotypic changes relative to non-transgenic controls. BAC-FUS-P525L mice have an early onset hyperactive phenotype on open field testing. FUS-P525L mCherry is mislocalized to the cytoplasm in primary motor neurons in vitro and in spinal cord tissue. These mice are considered 'high' expressers compared to Tg(FUS*P525L)1Kt mice (EMMA strain ID EM:13158). |
Breeding history | Founder mice (C57BL/6 x CBA)F1 were backcrossed to ~98% C57BL/6JOlaHsd (Harlan) and maintained on this background. |
References | None available |
Homozygous fertile | not known |
Homozygous viable | not known |
Homozygous matings required | no |
Immunocompromised | not known |
Information from EMMA
Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
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