B6.Cg-Trp63tm1.1Gml/Cnrm
| Status | Available to order |
| EMMA ID | EM:13212 |
| International strain name | B6.Cg-Trp63tm1.1Gml/Cnrm |
| Alternative name | Delta13Trp63+/- |
| Strain type | Targeted Mutant Strains : Other targeted |
| Allele/Transgene symbol | Trp63tm1.1Ozg, |
| Gene/Transgene symbol | Trp63 |
Information from provider
| Provider | Eleonora Candi |
| Provider affiliation | Experimental Medicine, University of Roma "Tor Vergata" |
| Genetic information | Heterozygous mice for deletion of exon 13 of Trp63 locus. Exon 13 was subjected to a cre-mediated deletion then the resulting allele is able to drive the expression of p63beta isoform instead of p63alpha. |
| Phenotypic information | Homozygous:Homozygous mice are not available.Heterozygous:Heterozygous females are sterile, so it was not possible to obtain a homozygous strain. Male mice are fertile. Other phenotypic features are under investigation. |
| Breeding history | Mice with Trp63 exon 13 floxed allele were generated by Ozgene (Ozgene Pty Ltd, Bentley, Australia), and the floxed/+ colony was amplified by crossing with wild-type C57BL/6J mice. Heterozygous floxed/+ mice were crossed with Tg(CMV-cre)1Cgn homozygous mice to obtain the p63 exon 13 deleted allele (Delta13p63). The Delta13p63/CMV-cre heterozygous mice were backcrossed with wild-type C57BL/6J mice to remove the CMV-cre allele. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- The p63 C-terminus is essential for murine oocyte integrity.;Lena Anna Maria, Rossi Valerio, Osterburg Susanne, Smirnov Artem, Osterburg Christian, Tuppi Marcel, Cappello Angela, Amelio Ivano, Dötsch Volker, De Felici Massimo, Klinger Francesca Gioia, Annicchiarico-Petruzzelli Margherita, Valensise Herbert, Melino Gerry, Candi Eleonora, ;2021;Nature communications;12;383; 33452256