STOCK Serpinf2tm1Hrl Tg(Alb-TTR*S52P)N4Psim/H
| Status | Available to order |
| EMMA ID | EM:13232 |
| Citation information | RRID:IMSR_EM:13232 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Serpinf2tm1Hrl Tg(Alb-TTR*S52P)N4Psim/H |
| Alternative name | STOCK Tg(Alb-TTR*S52P)N4Psim Serpinf2tm1Hrl |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Serpinf2tm1Hrl, Tg(Alb-TTR*S52P)N4Psim |
| Gene/Transgene symbol | Serpinf2, Tg(Alb-TTR*S52P)N4Psim |
Information from provider
| Provider | Paul Simons |
| Provider affiliation | Centre for Amyloidosis & Acute Phase Proteins, Division of Medicine, UCL |
| Additional owner | Prof. Dr. Lijnen H.R., Center for Molecular and Vascular Biology, Department of Cardiovascular Sciences, Leuven, Belgium |
| Genetic information | Transgenic for albumin enhancer/promoter-driven human transthyretin S52P amyloidogenic variant (TTR-S52P) gene and knock-out for alpha-2 antiplasmin (Serpinf2). |
| Phenotypic information | Homozygous:Phenotype of TTR-S52P transgenic homozygotes unknown.Heterozygous:Can be used to model human systemic transthyretin (ATTR) amyloidosis. Mice hemizygous for the TTR-S52P transgene express the S52P amyloidogenic variant of human TTR very abundantly (serum concentrations: 6.8 ± 0.8 mg/ml, males; 4.5 ± 1.0 mg/ml, females). TTR amyloid deposition can be seeded by i.v. injection of ex vivo ATTR amyloid. No TTR amyloid deposition has been observed in the absence of seeding with ex vivo ATTR amyloid. Seeded ATTR amyloid deposition is accelerated by homozygous alpha2-antiplasmin (Serpinf2) knock-out. TTR transgenic mice are smaller than non-transgenic littermates. |
| Breeding history | TTR transgene introduced by pronuclear microinjection in C57BL/6 x CBA embryos, backcrossed to C57BL/6. Alpha-2 antiplasmin (Serpinf2) knock-out allele generated in 129/SvJ ES cells, backcrossed to C57BL/6. Number of generations unknown. |
| References |
|
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Plasmin activity promotes amyloid deposition in a transgenic model of human transthyretin amyloidosis.;Slamova Ivana, Adib Rozita, Ellmerich Stephan, Golos Michal R, Gilbertson Janet A, Botcher Nicola, Canetti Diana, Taylor Graham W, Rendell Nigel, Tennent Glenys A, Verona Guglielmo, Porcari Riccardo, Mangione P Patrizia, Gillmore Julian D, Pepys Mark B, Bellotti Vittorio, Hawkins Philip N, Al-Shawi Raya, Simons J Paul, ;2021;Nature communications;12;7112; 34876572