IMPC phenotypes (gene matching)
B6.Cg-Tbcetm2c(EUCOMM)Hmgu/Ieg
| Status | Available to order |
| EMMA ID | EM:13235 |
| Citation information | RRID:IMSR_EM:13235 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tbcetm2c(EUCOMM)Hmgu/Ieg |
| Alternative name | TBCEtm2c (TBCEfl/fl) |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Tbcetm2c(EUCOMM)Hmgu |
| Gene/Transgene symbol | Tbce |
Information from provider
| Provider | Philip Wenzel |
| Provider affiliation | Cardiology 1, Center for Thrombosis and Hemostasis Mainz |
| Additional owner | TBCEtm2c founder mouse:
C57BL/6N-Tbce |
| Genetic information | Conditional knock-out of tubulin-folding cofactor E (Tbce) was achieved by using the 'flip knockout first approach' (EUCOMM strategy): C57BL/6N-Tbcetm2a(EUCOMM)Hmgu/Ieg mice (EMMA strain EM:08306; https://www.infrafrontier.eu/strain-search/?category=strains&keyword=Tbce) were crossed to flp recombinase-deleter mice. A subset of offspring carrying the tm2c allele (Tbce fl/wt) was outcrossed to wild-type C57BL/6N mice and floxed heterozygotes, negative for flp-deleter, were then chosen for brother x sister matings and subsequent line maintenance (Tbce fl/fl). |
| Phenotypic information | Homozygous:Tissue-specific Tbce knock-out in cre recombinase-expressing tissue can be achieved by crossing Tbce tm2c (Tbce fl/fl) to a cre recombinase-expressing mouse.Heterozygous:Tissue-specific Tbce knock-out in cre recombinase-expressing tissue can be achieved by crossing Tbce tm2c (Tbce fl/fl) to a cre recombinase-expressing mouse. |
| Breeding history | 8 generations of sibmatings have been performed on C57BL/6 background. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | homozygous C57BL/6N males |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Sanjad-Sakati syndrome / Orphanet_2323
- Autosomal recessive Kenny-Caffey syndrome / Orphanet_93324
- Early-onset progressive encephalopathy-spastic ataxia-distal spinal muscular atrophy syndrome / Orphanet_496756
Literature references
- Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress.;Efentakis Panagiotis, Molitor Michael, Kossmann Sabine, Bochenek Magdalena L, Wild Johannes, Lagrange Jeremy, Finger Stefanie, Jung Rebecca, Karbach Susanne, Schäfer Katrin, Schulz Andreas, Wild Philipp, Münzel Thomas, Wenzel Philip, ;2022;European heart journal;43;488-500; 34132336