129(Cg)-Igktm3.1Cog/Orl
| Status | Available to order |
| EMMA ID | EM:13237 |
| Citation information | RRID:IMSR_EM:13237 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129(Cg)-Igktm3.1Cog/Orl |
| Alternative name | c3'RR-KIKS |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Igktm3.1Cog |
| Gene/Transgene symbol | Igk |
Information from provider
| Provider | Sandrine Le Noir |
| Provider affiliation | CNRS UMR 7276 |
| Genetic information | The strategy used to generate c3’RR-KIKS mice was identical to that previously described for KIKS mice (EMMA strain ID EM:11249; PMID: 26146363), except for the inclusion of a c3’RR cassette downstream of the genomic fragment containing the Ei enhancer and Cgene. The c3’RR cassette included all four 3’RR enhancers in their normal palindromic layout, as described (PMID: 28369649 ). |
| Phenotypic information | Homozygous:DNA lesions instrumentally inflicted by activation-induced deaminase (AID) initiate the processes reshaping immunoglobulin genes in mature B-cells, from local somatic hypermutation (SHM) to junctions of distant breaks during class switch recombination (CSR). It remains incompletely understood how these divergent outcomes of AID attacks are differentially and temporally focused, with CSR strictly occurring in the Ig heavy chain (IgH) locus while SHM concentrates on rearranged V(D)J regions in the IgH and Ig light chain loci. In the IgH locus, disruption of either the 3’Regulatory Region (3’RR) super-enhancer or of switch (S) regions preceding constant genes, profoundly affects CSR. Reciprocally, we have examined if these elements are sufficient to induce CSR in a synthetic locus based on the Igk locus backbone. Addition of a surrogate “core 3’RR” (c3’RR) and of a pair of transcribed and spliced Switch regions, together with a reporter system for “k-CSR” yielded a switchable Igk locus.Heterozygous:NA |
| Breeding history | After germline transmission, mice were bred with a cre-expressing strain (CMV-cre) to delete the NeoR cassette and mice were then bred together. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | homozygous 129 males |
Literature references
- IgH 3' regulatory region increases ectopic class switch recombination.;Le Noir Sandrine, Bonaud Amélie, Hervé Bastien, Baylet Audrey, Boyer François, Lecardeur Sandrine, Oruc Zeliha, Sirac Christophe, Cogné Michel, ;2021;PLoS genetics;17;e1009288; 33556079