B6.Cg-Col1a1tm1(tetO-Cpt1a,-GFP)Slms Tg(Pax8-rtTA2S*M2)1Koes/Cnbc

Status

Available to order

EMMA IDEM:13348
Citation informationRRID:IMSR_EM:13348 

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International strain nameB6.Cg-Col1a1tm1(tetO-Cpt1a,-GFP)Slms Tg(Pax8-rtTA2S*M2)1Koes/Cnbc
Alternative namePax8-rtTAtg/0:tetO-Cpt1atg/0 (Pax8-CPT1A)
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolCol1a1tm1(tetO-Cpt1a,-GFP)Slms, Tg(Pax8-rtTA2S*M2)1Koes
Gene/Transgene symbolCol1a1, Tg(Pax8-rtTA2S*M2)1Koes

Information from provider

ProviderSantiago Lamas
Provider affiliationProcesos Fisiológicos y Patológicos, Centro de Biología Molecular Severo Ochoa
Genetic informationPax8-rtTA cassette (Chr 8): expression of rtTA in renal proximal epithelial cells. TRE-CPT1A-P2A-GFP cassette (Col1a1 locus): CPT1A and GFP coding sequence under the control of the TRE element. P2A allows the protein excision among CPT1A and GFP. The TRE-CPT1A-PA2A-GFP cassette (minimal CMV promoter) contained in a pBS31 vector was inserted downstream of the Col1a1 locus by FRT/flippase-mediated recombination.
Phenotypic informationHomozygous:
Phenotypically undistinguishable from wild-type mice. No anatomical anomalies, physiological variations, behavioural abnormalities or food uptake alterations.

Heterozygous:
No phenotypic alterations. Mice present a CPT1A overexpression and enhanced fatty acid oxidation in the tubular cells in the kidney.
Breeding historyPax8-rtTA mice (C57BL/6J; JAX number 007176) were crossed with the newly generated TRE-CPT1A mice (B6N;129/Sv background). Resulting mice were backcrossed to C57BL/6J mice for at least 5 generations to generate this congenic strain.
References
  • Renal tubule Cpt1a overexpression protects from kidney fibrosis by restoring mitochondrial homeostasis.;Miguel Verónica, Tituaña Jessica, Herrero J Ignacio, Herrero Laura, Serra Dolors, Cuevas Paula, Barbas Coral, Puyol Diego Rodríguez, Márquez-Expósito Laura, Ruiz-Ortega Marta, Castillo Carolina, Sheng Xin, Susztak Katalin, Ruiz-Canela Miguel, Salas-Salvadó Jordi, González Miguel A Martínez, Ortega Sagrario, Ramos Ricardo, Lamas Santiago, ;2021;The Journal of clinical investigation;131;; 33465052
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous C57BL/6J males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (gene matching)
  • abnormal adipose tissue distribution / MGI
  • big ears / MGI
  • delayed bone ossification / MGI
  • fragile skeleton / MGI
  • increased bone mineral density / MGI
  • decreased bone mineral density / MGI
  • failure of bone resorption / MGI
  • osteopetrosis / MGI
  • kyphoscoliosis / MGI
  • abnormal neurocranium morphology / MGI
  • decreased compact bone thickness / MGI
  • abnormal vertebral body morphology / MGI
  • abnormal rib morphology / MGI
  • kyphosis / MGI
  • scoliosis / MGI
  • heart right ventricle hypertrophy / MGI
  • increased cell proliferation / MGI
  • abnormal cell morphology / MGI
  • focal hair loss / MGI
  • abnormal cranium morphology / MGI
  • abnormal maxilla morphology / MGI
  • abnormal digestive system morphology / MGI
  • abnormal forelimb morphology / MGI
  • abnormal femur morphology / MGI
  • syndactyly / MGI
  • oligodactyly / MGI
  • abnormal autopod morphology / MGI
  • thin tail / MGI
  • abnormal liver morphology / MGI
  • weakness / MGI
  • hindlimb paralysis / MGI
  • abnormal uterus morphology / MGI
  • lung hemorrhage / MGI
  • thick skin / MGI
  • skin hyperplasia / MGI
  • abnormal dermal layer morphology / MGI
  • thick dermal layer / MGI
  • increased body length / MGI
  • decreased body length / MGI
  • increased body weight / MGI
  • decreased body weight / MGI
  • increased body size / MGI
  • decreased body size / MGI
  • abnormal tail movements / MGI
  • abnormal locomotor behavior / MGI
  • hypoactivity / MGI
  • abnormal gait / MGI
  • abnormal motor coordination/balance / MGI
  • abnormal osteoclast physiology / MGI
  • abnormal cardiovascular system physiology / MGI
  • abnormal blood vessel morphology / MGI
  • embryonic growth arrest / MGI
  • lung inflammation / MGI
  • hemorrhage / MGI
  • intracranial hemorrhage / MGI
  • increased litter size / MGI
  • decreased litter size / MGI
  • delayed sexual maturation / MGI
  • respiratory distress / MGI
  • abnormal coat/hair pigmentation / MGI
  • premature death / MGI
  • abnormal limb morphology / MGI
  • abnormal digit morphology / MGI
  • abnormal limb bone morphology / MGI
  • abnormal fertility/fecundity / MGI
  • no abnormal phenotype detected / MGI
  • abnormal fibula morphology / MGI
  • abnormal turbinate morphology / MGI
  • decreased susceptibility to hepatic steatosis / MGI
  • abnormal blood gas level / MGI
  • delayed vaginal opening / MGI
  • decreased circulating insulin level / MGI
  • abnormal mechanical nociception / MGI
  • decreased vertical activity / MGI
  • abnormal dental pulp cavity morphology / MGI
  • coarse hair / MGI
  • increased growth rate / MGI
  • increased insulin sensitivity / MGI
  • abnormal bone mineralization / MGI
  • increased circulating parathyroid hormone level / MGI
  • decreased circulating alkaline phosphatase level / MGI
  • arthritis / MGI
  • abnormal bone healing / MGI
  • no phenotypic analysis / MGI
  • decreased skin tensile strength / MGI
  • decreased tendon stiffness / MGI
  • short femur / MGI
  • decreased susceptibility to age related obesity / MGI
  • aneurysm / MGI
  • osteoarthritis / MGI
  • abnormal long bone morphology / MGI
  • abnormal compact bone morphology / MGI
  • abnormal forelimb zeugopod morphology / MGI
  • increased hepatocyte proliferation / MGI
  • abnormal hormone level / MGI
  • increased lean body mass / MGI
  • decreased thermal nociceptive threshold / MGI
  • decreased bone mass / MGI
  • aortic dissection / MGI
  • abnormal chest morphology / MGI
  • abnormal pubis morphology / MGI
  • abnormal ischium morphology / MGI
  • abnormal pelvic girdle bone morphology / MGI
  • thin ribs / MGI
  • rib fractures / MGI
  • decreased length of long bones / MGI
  • abnormal vertebral column morphology / MGI
  • short vertebral body / MGI
  • increased susceptibility to age-related hearing loss / MGI
  • abnormal osteoblast morphology / MGI
  • increased osteoblast cell number / MGI
  • decreased bone strength / MGI
  • increased bone resorption / MGI
  • abnormal osteoblast physiology / MGI
  • liver hyperplasia / MGI
  • decreased susceptibility to injury / MGI
  • ocular hypertension / MGI
  • improved glucose tolerance / MGI
  • abnormal humerus morphology / MGI
  • abnormal enzyme/coenzyme level / MGI
  • abnormal myocardium layer morphology / MGI
  • homeostasis/metabolism phenotype / MGI
  • hearing/vestibular/ear phenotype / MGI
  • digestive/alimentary phenotype / MGI
  • reproductive system phenotype / MGI
  • skeleton phenotype / MGI
  • vision/eye phenotype / MGI
  • decreased percent body fat/body weight / MGI
  • abnormal skeleton morphology / MGI
  • decreased circulating glucose level / MGI
  • decreased ventricle muscle contractility / MGI
  • decreased susceptibility to diet-induced obesity / MGI
  • spinal hemorrhage / MGI
  • abnormal heart septum morphology / MGI
  • impaired hearing / MGI
  • decreased diameter of femur / MGI
  • abnormal myometrium morphology / MGI
  • abnormal osteoblast differentiation / MGI
  • abnormal cutaneous collagen fibril morphology / MGI
  • abnormal osteocyte morphology / MGI
  • fused carpal bones / MGI
  • delayed female fertility / MGI
  • small pancreatic islets / MGI
  • decreased trabecular bone thickness / MGI
  • abnormal endoplasmic reticulum morphology / MGI
  • abnormal aorta elastic tissue morphology / MGI
  • abnormal aorta tunica adventitia morphology / MGI
  • decreased total body fat amount / MGI
  • abnormal thoracic cage shape / MGI
  • decreased bone mineral content / MGI
  • abnormal heart electrocardiography waveform feature / MGI
  • myocardial hypertrophy / MGI
  • increased QRS amplitude / MGI
  • abnormal bone trabecula morphology / MGI
  • decreased bone trabecula number / MGI
  • decreased bone volume / MGI
  • decreased trabecular bone volume / MGI
  • pulmonary alveolar hemorrhage / MGI
  • abnormal compact bone lamellar structure / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • neonatal lethality, complete penetrance / MGI
  • neonatal lethality, incomplete penetrance / MGI
  • prenatal lethality, complete penetrance / MGI
  • embryonic lethality, complete penetrance / MGI
  • embryonic lethality during organogenesis, complete penetrance / MGI
  • preweaning lethality, incomplete penetrance / MGI
  • abnormal bone collagen fibril morphology / MGI
  • abnormal tendon collagen fibril morphology / MGI
  • decreased neuronal stem cell self-renewal / MGI
  • thin body / MGI
  • abnormal extracellular matrix morphology / MGI
  • osteophytes / MGI
  • increased bone trabecular spacing / MGI
  • decreased bone trabecular spacing / MGI
  • decreased bone ossification / MGI
  • decreased bone mineralization / MGI
  • decreased collagen level / MGI
  • broad face / MGI
  • thick neurocranium / MGI
  • thin neurocranium / MGI
  • increased osteoblast apoptosis / MGI
  • abnormal dentin mineralization / MGI
  • increased osteocyte apoptosis / MGI
  • abnormal osteocyte lacuna morphology / MGI
  • joint contracture / MGI
  • atlanto-occipital joint dislocation / MGI
  • abnormal olecranon morphology / MGI

Literature references

  • Renal tubule Cpt1a overexpression protects from kidney fibrosis by restoring mitochondrial homeostasis.;Miguel Verónica, Tituaña Jessica, Herrero J Ignacio, Herrero Laura, Serra Dolors, Cuevas Paula, Barbas Coral, Puyol Diego Rodríguez, Márquez-Expósito Laura, Ruiz-Ortega Marta, Castillo Carolina, Sheng Xin, Susztak Katalin, Ruiz-Canela Miguel, Salas-Salvadó Jordi, González Miguel A Martínez, Ortega Sagrario, Ramos Ricardo, Lamas Santiago, ;2021;The Journal of clinical investigation;131;; 33465052

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  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
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