129S2(Cg)-Trp53tm1.1Thst/Orl
| Status | Available to order |
| EMMA ID | EM:13422 |
| International strain name | 129S2(Cg)-Trp53tm1.1Thst/Orl |
| Alternative name | 129S2-Trp53 tm1.1Thst/ Thst |
| Strain type | Targeted Mutant Strains : Point mutation |
| Allele/Transgene symbol | Trp53tm1.1Thst, |
| Gene/Transgene symbol | Trp53 |
Information from provider
| Provider | Thorsten Stiewe |
| Provider affiliation | Institute of Molecular Oncology, Philipps University Marburg |
| Genetic information | The endogenous Trp53 allele carries a point mutation GAG->CGG in the exon 5 resulting in Glu->Arg substitution in the codon 177. |
| Phenotypic information | Homozygous:E177R substitution leads to reduced p53 DNA binding cooperativity, which results in decreased transcriptional activation of target genes and deficiency in p53-dependent transcription-mediated apoptosis. Homozygous mice are resistant to gamma-irradiation induced apoptosis, are cancer-prone (B-cell and T-cell lymphoma, angiosarcoma, carcinoma; 21% develop spleen hyperplasia) and have a reduced lifespan (median overall survival of 361 days, whereas the lifespan of the p53+/+ littermates exceeded 770 days).Heterozygous:Heterozygous p53+/E177R mice are tumor-prone and have reduced lifespan (median overall survival 587 days). Among all neoplasms detected in heterozygous mice the frequency of non-malignant tumors (predominantly spleen hyperplasia) reaches 50%, malignant tumors are represented by B-cell lymphoma, sarcoma/angiosarcoma and carcinoma. |
| Breeding history | More than 20 backcrosses to 129S2/SvHsd background. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous 129S/SvHsd, wild-type 129S/SvHsd |
| Stage of embryos | 2-cell |
Literature references
- Inactivation of Mdm2 restores apoptosis proficiency of cooperativity mutant p53 in vivo.;Klimovich Boris, Stiewe Thorsten, Timofeev Oleg, ;2020;Cell cycle (Georgetown, Tex.);19;109-123; 31749402
- p53 DNA binding cooperativity is essential for apoptosis and tumor suppression in vivo.;Timofeev Oleg, Schlereth Katharina, Wanzel Michael, Braun Attila, Nieswandt Bernhard, Pagenstecher Axel, Rosenwald Andreas, Elsässer Hans-Peter, Stiewe Thorsten, ;2013;Cell reports;3;1512-25; 23665223