129S2(Cg)-Trp53tm2.1Thst/Orl
| Status | Available to order |
| EMMA ID | EM:13424 |
| International strain name | 129S2(Cg)-Trp53tm2.1Thst/Orl |
| Alternative name | 129S2-Trp53 |
| Strain type | Targeted Mutant Strains : Point mutation |
| Allele/Transgene symbol | Trp53tm2.1Thst, |
| Gene/Transgene symbol | Trp53 |
Information from provider
| Provider | Thorsten Stiewe |
| Provider affiliation | Molecular Oncology Institute, Philipps University Marburg |
| Genetic information | The endogenous Trp53 allele carries a point mutation CGC->GAG in the exon 5 resulting in Arg->Glu substitution in the codon 178 |
| Phenotypic information | Homozygous:R178E substitution leads to severely compromised p53 DNA binding cooperativity, which results in loss of transcriptional activation of direct target genes and deficiency in p53-dependent transcription-mediated functions. Homozygous mice are resistant to gamma-irradiation induced apoptosis, are cancer-prone (tumor spectrum is very close to p53 knock-out - about 90% of tumors are T- and B-lymphoma) and have a strongly reduced lifespan (median overall survival of knock-ins 150 days, knock-outs 129 days, whereas the lifespan of the p53+/+ littermates exceeded 842 days).Heterozygous:Heterozygous p53+/R178E mice are tumor-prone and have reduced lifespan (median overall survival 519 days). The most frequent malignant tumors are sarcoma (47%), lymphoma (29%), and carcinoma (12%). |
| Breeding history | More than 20 backcrosses on 129S2/SvHsd background |
| References |
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| Homozygous fertile | males only |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous 129S/SvHsd |
Literature references
- Residual apoptotic activity of a tumorigenic p53 mutant improves cancer therapy responses.;Timofeev Oleg, Klimovich Boris, Schneikert Jean, Wanzel Michael, Pavlakis Evangelos, Noll Julia, Mutlu Samet, Elmshäuser Sabrina, Nist Andrea, Mernberger Marco, Lamp Boris, Wenig Ulrich, Brobeil Alexander, Gattenlöhner Stefan, Köhler Kernt, Stiewe Thorsten, ;2019;The EMBO journal;38;e102096; 31483066