129S2(Cg)-Trp53tm3.1Thst/Orl
| Status | Available to order |
| EMMA ID | EM:13426 |
| International strain name | 129S2(Cg)-Trp53tm3.1Thst/Orl |
| Alternative name | 129S2-Trp53 |
| Strain type | Targeted Mutant Strains : Point mutation |
| Allele/Transgene symbol | Trp53tm3.1Thst, |
| Gene/Transgene symbol | Trp53 |
Information from provider
| Provider | Thorsten Stiewe |
| Provider affiliation | Molecular Oncology Institute, Philipps University Marburg |
| Genetic information | The endogenous Trp53 allele carries a point mutation TCC->GCC in the exon 5 resulting in Ser->Ala substitution in the codon 180 |
| Phenotypic information | Homozygous:Homozygous S180A mice are tumor resistant but have slightly shortened lifespan. For example, S180A knock-in mice showed markedly reduced spontaneous tumorigenesis and increased resistance to Myc-driven lymphoma and Eml4-Alk-driven lung cancer. Median lifespan was significantly reduced from 709 days in wild type to 568 days in S180A littermates. Lifespan was reduced by a loss of general fitness and increased susceptibility to age-related diseases.Heterozygous:Heterozygous mice have normal phenotype |
| Breeding history | More than 20 backcrosses on 129S2/SvHsd background |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous 129S/SvHsd, wild-type 129S/SvHsd |
| Stage of embryos | 2-cell |
Literature references
- Phosphorylation Control of p53 DNA-Binding Cooperativity Balances Tumorigenesis and Aging.;Timofeev Oleg, Koch Lukas, Niederau Constantin, Tscherne Alina, Schneikert Jean, Klimovich Maria, Elmshäuser Sabrina, Zeitlinger Marie, Mernberger Marco, Nist Andrea, Osterburg Christian, Dötsch Volker, Hrabé de Angelis Martin, Stiewe Thorsten, null null, ;2020;Cancer research;80;5231-5244; 32873634