B6;CBA-Tg(tetO-lacZ,-SHANK2_iA,-Venus)1Rsp/Ieg
| Status | Available to order |
| EMMA ID | EM:13462 |
| Citation information | RRID:IMSR_EM:13462 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;CBA-Tg(tetO-lacZ,-SHANK2_iA,-Venus)1Rsp/Ieg |
| Alternative name | TgSHANK2A |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(tetO-lacZ,-SHANK2_iA,-Venus)1Rsp |
| Gene/Transgene symbol | Tg(tetO-lacZ,-SHANK2_iA,-Venus)1Rsp |
Information from provider
| Provider | Rolf Sprengel |
| Provider affiliation | Working Group Molecular Neurobiology, Max Planck Institute for Medical Research |
| Genetic information | Tetracycline-controlled transgene for the SHANK2A overexpression. The transgene contains a bidirectional tTA-dependent expression cassette which encodes on one site the nuclear localized beta-galactosidase and on the other side the Venus (green fluorescent protein variant) reporter-T2A ribosomal skipping sequence-SHANK2A fusion. The sequence origin for SHANK2A is human. |
| Phenotypic information | Homozygous:unknownHeterozygous:No phenotype in absence of the transcriptional activator TA. Upon crossing with TgCam2a-tTA mice, the TgSHANK2A/tTA double-transgenic animals develop an autism spectrum disorder-like phenotype, have a reduced body weight and about 25% show a premature death. |
| Breeding history | The line was generated in November 2013 and intercrossed routinely with TgCam2A-tTA mice (B6;CBA-Tg(Camk2a-tTA)1Mmay/J; Stock No: 003010). Double transgenic mice were never used for matings. Heterozygous TgSHANK2A mice were used together with C57BL/6 mice to keep the line. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6N males |
Literature references
- Imbalanced post- and extrasynaptic SHANK2A functions during development affect social behavior in SHANK2-mediated neuropsychiatric disorders.;Eltokhi Ahmed, Gonzalez-Lozano Miguel A, Oettl Lars-Lennart, Rozov Andrey, Pitzer Claudia, Röth Ralph, Berkel Simone, Hüser Markus, Harten Aliona, Kelsch Wolfgang, Smit August B, Rappold Gudrun A, Sprengel Rolf, ;2021;Molecular psychiatry;26;6482-6504; 34021263