B6;CBA-Tg(tetO-lacZ,-SHANK2_iA*,-Venus)2Rsp/Ieg
| Status | Available to order |
| EMMA ID | EM:13463 |
| Citation information | RRID:IMSR_EM:13463 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;CBA-Tg(tetO-lacZ,-SHANK2_iA*,-Venus)2Rsp/Ieg |
| Alternative name | TgSHANK2A(R462X) |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(tetO-lacZ,-SHANK2_iA*,-Venus)2Rsp |
| Gene/Transgene symbol | Tg(tetO-lacZ,-SHANK2_iA*,-Venus)2Rsp |
Information from provider
| Provider | Rolf Sprengel |
| Provider affiliation | Molecular Neurobiology, Max Planck Institute for Medical Research |
| Genetic information | Tetracycline-controlled transgenic overexpression for the SHANK2A(R462X) truncated mutation, that was found in human autism spectrum disorder (ASD) patients. The transgene contains a bidirectional tTA-dependent expression cassette which encodes on one site the nuclear localized beta-galactosidase and on the other side the VENUS-tTA-SHANK2A(R462X) fusion. The sequence origin for SHANK2A(R462X) is human. |
| Phenotypic information | Homozygous:UnknownHeterozygous:No phenotype in absence of the doxycycline doxycycline sensitive transcriptional activator tTA. |
| Breeding history | The line was generated in November 2013. Subsequently the line was intercrossed routinely with TgCam2a-tTA mice (B6;CBA-Tg(Camk2a-tTA)1Mmay/J - JAXMice Stock No: 003010). Double transgenic TgSHANK2A(R462X)/Cam2a-tTA mice were never used for matings. Hemizygous TgSHANK2A(R462X) mice were backcrossed to C57BL/6N mice to keep the line. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6N males |
Literature references
- Imbalanced post- and extrasynaptic SHANK2A functions during development affect social behavior in SHANK2-mediated neuropsychiatric disorders.;Eltokhi Ahmed, Gonzalez-Lozano Miguel A, Oettl Lars-Lennart, Rozov Andrey, Pitzer Claudia, Röth Ralph, Berkel Simone, Hüser Markus, Harten Aliona, Kelsch Wolfgang, Smit August B, Rappold Gudrun A, Sprengel Rolf, ;2021;Molecular psychiatry;26;6482-6504; 34021263