B6NCrl.Cg-Uqcrhtm1b(EUCOMM)Wtsi Gt(ROSA)26Sortm1.1(CAG-AOX)Htj/Ieg
| Status | Available to order |
| EMMA ID | EM:13642 |
| International strain name | B6NCrl.Cg-Uqcrhtm1b(EUCOMM)Wtsi Gt(ROSA)26Sortm1.1(CAG-AOX)Htj/Ieg |
| Alternative name | Uqox_het |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Gt(ROSA)26Sortm1.1(CAG-AOX)Htj, |
| Gene/Transgene symbol | Gt(ROSA)26Sor |
Information from provider
| Provider | Valerie Gailus-Durner |
| Provider affiliation | GMC, IEG |
| Additional owner | Uqox belongs to Dr. Valerie Gailus Durner; AOXrosa26 belongs to Dr. Marten Szibor, Jena University Hospital, Germany; Uqcrh belongs to Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Germany. |
| Genetic information | Uqcrh_EPD0378_3_C07_tm1b-het + Gt(ROSA)26Sor_AOX_KI-het. Uqcrh-mutant mice were generated on a C57BL/6NCrl genetic background and maintained by het x wild-type maiting over >5 generations. Gt(ROSA)26Sor_AOX_KI (a.k.a. AOX-Rosa26-tg; MGI:5824337 allele ID) mice were originally generated and maintained on a C57BL/6J genetic background. Heterozygous animals were outcrossed to C57BL/6NCrl wild-type animals over seven generations, before the parental generation for the production of Uqox animals was generated (see below). Uqox mice were produced by mating in a first step C57BL/6NCrl Gt(ROSA)26Sor_AOX_KI heterozygous mice (AOX het) to C57BL/6NCrl Uqcrh-tm1b (MGI:5692661 allele ID) heterozygous animals (Uqcrh het) to produce double heterozygous animals. For preservation of the line sperm of Uqcrh het/AOX het males was used. In a second step Uqcrh het/AOX het offspring is mated to Uqcrh het/AOX wild-type offspring to produce Uqcrh hom/AOX het animals (so-called Uqox mice). |
| Phenotypic information | Homozygous:Uqcrh_hom: low body weight; small; severe hyperglycemia from 6 weeks of age; reduced livespan (max. 11 weeks).Heterozygous:Uqcrh_het: none |
| Breeding history | Gt(ROSA)26Sor_AOX_KI-het x Uqcrh_EPD0378_3_C07_tm1b-het. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6N |
Literature references
- AOX delays the onset of the lethal phenotype in a mouse model of Uqcrh (complex III) disease;Jacobs HT, Szibor M, Rathkolb B, da Silva-Buttkus P, Aguilar-Pimentel JA, Amarie OV, Becker L, Calzada-Wack J, Dragano N, Garrett L, Gerlini R, Hölter SM, Klein-Rodewald T, Kraiger M, Leuchtenberger S, Marschall S, Östereicher MA, Pfannes K, Sanz-Moreno A, Seisenberger C, Spielmann N, Stoeger C, Wurst W, Fuchs H, Hrabe de Angelis M, Gailus-Durner V.;2023;Biochim Biophys Acta Mol Basis Dis;1869;166760; 37230398