C57BL/6N-Cdk6tm1c(EUCOMM)Wtsi/TcpBiat

Status

Available to order

EMMA IDEM:13810
Citation informationRRID:IMSR_EM:13810 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Cdk6tm1c(EUCOMM)Wtsi/TcpBiat
Alternative nameC57BL/6N-Cdk6 tm1c(EUCOMM)Wtsi/Tcp
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolCdk6tm1c(EUCOMM)Wtsi
Gene/Transgene symbolCdk6

Information from provider

ProviderVeronika Sexl
Provider affiliationDepartment of Biomedical Sciences, University of Veterinary Medicine
Genetic informationThe mouse line C57BL/6N-Cdk6tm1c(EUCOMM)Wtsi/Tcp (referred to as Cdk6fl/fl) was generated with C57BL/6N-Cdk6tm1a(EUCOMM)Wtsi/Tcp (available from the Canadian Mouse Mutant Repository-CMMR at http://www.cmmr.ca/order.php?t=m&id=1766) made from EUCOMM ES cells (Bradley A, Anastassiadis K, Ayadi A, et al. The mammalian gene function resource: the International Knockout Mouse Consortium. Mamm Genome. 2012;23:580-586) at the Toronto Centre for Phenogenomics, Canada.
Phenotypic informationHomozygous:
No data available; no phenotype expected.

Heterozygous:
No data available; no phenotype expected.
Breeding historyThe flp recombinase-mediated excision was obtained by breeding a C57BL/6N-Cdk6tm1a(EUCOMM)Wtsi/Tcp heterozygous male with heterozygous C57BL/6N-Gt(ROSA)26Sortm2(CAG-flpo,-EYFP)Ics/Tcp female(s). The mouse line was established by backcrossing Cdk6tm1c+/-; flp+/- mice to C57BL/6NCrl (less than 10 generations).
References
  • Inducible deletion of CDK4 and CDK6 - deciphering CDK4/6 inhibitor effects in the hematopoietic system.;Maurer Barbara, Brandstoetter Tania, Kollmann Sebastian, Sexl Veronika, Prchal-Murphy Michaela, ;2021;Haematologica;106;2624-2632; 32855282
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreUniversity of Veterinary Medicine, Vienna, Austria
Animals used for archivinghomozygous C57BL/6N males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • abnormal eye morphology / IMPC
  • increased mean corpuscular volume / IMPC
  • small superior vagus ganglion / IMPC
  • increased mean corpuscular hemoglobin / IMPC
MGI phenotypes (gene matching)
  • decreased leukocyte cell number / MGI
  • abnormal spleen morphology / MGI
  • small spleen / MGI
  • spleen hypoplasia / MGI
  • abnormal thymus morphology / MGI
  • small thymus / MGI
  • enlarged thymus / MGI
  • increased thymocyte number / MGI
  • decreased thymocyte number / MGI
  • decreased body weight / MGI
  • thymus hypoplasia / MGI
  • reduced female fertility / MGI
  • abnormal definitive hematopoiesis / MGI
  • abnormal T cell differentiation / MGI
  • abnormal spleen red pulp morphology / MGI
  • abnormal hematopoietic system morphology/development / MGI
  • abnormal lymphopoiesis / MGI
  • abnormal erythrocyte morphology / MGI
  • increased mean corpuscular volume / MGI
  • decreased mature ovarian follicle number / MGI
  • decreased erythrocyte cell number / MGI
  • thymus atrophy / MGI
  • abnormal thymocyte activation / MGI
  • increased hematopoietic stem cell number / MGI
  • decreased hematopoietic stem cell number / MGI
  • decreased double-negative T cell number / MGI
  • increased double-negative T cell number / MGI
  • decreased double-positive T cell number / MGI
  • decreased T cell proliferation / MGI
  • immune system phenotype / MGI
  • increased mean corpuscular hemoglobin concentration / MGI
  • increased T cell apoptosis / MGI
  • increased CD4-positive, alpha beta T cell number / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • increased single-positive T cell number / MGI
  • decreased megakaryocyte cell number / MGI
  • decreased thymocyte apoptosis / MGI
  • increased DN1 thymic pro-T cell number / MGI
  • decreased DN1 thymic pro-T cell number / MGI
  • decreased DN2 thymocyte number / MGI
  • increased DN3 thymocyte number / MGI
  • decreased DN4 thymocyte number / MGI
  • abnormal hematopoietic stem cell physiology / MGI
  • thymus cortex atrophy / MGI

Literature references

  • Inducible deletion of CDK4 and CDK6 - deciphering CDK4/6 inhibitor effects in the hematopoietic system.;Maurer Barbara, Brandstoetter Tania, Kollmann Sebastian, Sexl Veronika, Prchal-Murphy Michaela, ;2021;Haematologica;106;2624-2632; 32855282

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

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Legally binding conditions for the transfer

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