STOCK Dll1^em1.2Gos/Orl

Status	Available to order
EMMA ID	EM:13832
Citation information	RRID:IMSR_EM:13832 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	STOCK Dll1^em1.2Gos/Orl
Alternative name	Dll1SF
Strain type	Endonuclease-mediated
Allele/Transgene symbol	Dll1^em1.2Gos
Gene/Transgene symbol	Dll1

Information from provider

Provider	Achim Gossler
Provider affiliation	Molekularbiologie, MHH
Genetic information	Dll1 gene modified such that the protein carries a C-terminal Strep/FLAG (SF) tag. CRISPR/Cas9 used to induce double strand breaks at the locus.
Phenotypic information	Homozygous: Short and kinky tails as a consequence of somite patterning defects. Viable; 60% of males were infertile. Heterozygous: No known phenotype
Breeding history	Kept for more than 10 generations on a mixed 129S1/SvImJ, CD1, C57BL/6 background
References	Activity of the mouse Notch ligand DLL1 is sensitive to C-terminal tagging in vivo.;Schuster-Gossler Karin, Boldt Karsten, Bornhorst Dorothee, Delany-Heiken Patricia, Ueffing Marius, Gossler Achim, ;2021;BMC research notes;14;383; 34583743
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France
Animals used for archiving	heterozygous (EM:13832 backg., mixed 129S1/SvImJ, CD1, C57BL/6) males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Alobar holoprosencephaly / Orphanet_93925
Autosomal dominant non-syndromic intellectual disability / Orphanet_178469
Midline interhemispheric variant of holoprosencephaly / Orphanet_93926
Microform holoprosencephaly / Orphanet_280200
Septopreoptic holoprosencephaly / Orphanet_280195
Semilobar holoprosencephaly / Orphanet_220386
Lobar holoprosencephaly / Orphanet_93924

IMPC phenotypes (gene matching)

increased monocyte cell number / IMPC
abnormal whole-body plethysmography / IMPC
decreased tidal volume / IMPC
abnormal behavior / IMPC
increased blood urea nitrogen level / IMPC
decreased body weight / IMPC
increased hemoglobin content / IMPC
decreased circulating calcium level / IMPC
decreased body temperature / IMPC
decreased circulating cholesterol level / IMPC
thrombocytopenia / IMPC
decreased circulating triglyceride level / IMPC
abnormal anxiety-related response / IMPC
decreased mean platelet volume / IMPC
decreased circulating glucose level / IMPC
decreased vertical activity / IMPC
increased leukocyte cell number / IMPC
decreased circulating potassium level / IMPC
hyperactivity / IMPC
decreased defecation amount / IMPC
decreased circulating sodium level / IMPC
increased lymphocyte cell number / IMPC
increased grip strength / IMPC
abnormal righting response / IMPC
decreased circulating chloride level / IMPC
increased granulocyte number / IMPC
abnormal eye electrophysiology / IMPC

MGI phenotypes (gene matching)

increased bone mineral density / MGI
decreased bone mineral density / MGI
abnormal vertebrae morphology / MGI
abnormal heart morphology / MGI
short tail / MGI
abnormal myogenesis / MGI
abnormal brain development / MGI
fused dorsal root ganglion / MGI
abnormal spinal nerve morphology / MGI
decreased body height / MGI
decreased body length / MGI
decreased body weight / MGI
hyperactivity / MGI
hypoactivity / MGI
no spontaneous movement / MGI
abnormal blood vessel morphology / MGI
abnormal somite development / MGI
incomplete somite formation / MGI
abnormal somite shape / MGI
decreased IgA level / MGI
hemorrhage / MGI
perinatal lethality / MGI
premature death / MGI
abnormal muscle morphology / MGI
abnormal axial skeleton morphology / MGI
abnormal neural tube morphology / MGI
no abnormal phenotype detected / MGI
decreased circulating triglyceride level / MGI
abnormal somatic nervous system morphology / MGI
situs inversus / MGI
abnormal notochord morphology / MGI
nervous system phenotype / MGI
abnormal defecation / MGI
abnormal pancreas development / MGI
increased lean body mass / MGI
decreased lean body mass / MGI
abnormal primitive node morphology / MGI
caudal body truncation / MGI
absent embryonic cilia / MGI
abnormal direction of embryo turning / MGI
abnormal direction of heart looping / MGI
decreased lumbar vertebrae number / MGI
decreased skeletal muscle mass / MGI
increased mean systemic arterial blood pressure / MGI
decreased B-1 B cell number / MGI
decreased neuronal precursor cell number / MGI
increased B cell number / MGI
decreased circulating cholesterol level / MGI
increased motor neuron number / MGI
decreased heart rate / MGI
muscle phenotype / MGI
embryo phenotype / MGI
immune system phenotype / MGI
decreased circulating total protein level / MGI
abnormal enzyme/coenzyme activity / MGI
artery stenosis / MGI
increased systemic arterial diastolic blood pressure / MGI
increased systemic arterial systolic blood pressure / MGI
abnormal paraxial mesoderm morphology / MGI
decreased CD4-positive, alpha beta T cell number / MGI
increased CD8-positive, alpha-beta T cell number / MGI
decreased CD8-positive, alpha-beta T cell number / MGI
decreased IgG1 level / MGI
decreased IgG2b level / MGI
decreased IgG3 level / MGI
increased blood uric acid level / MGI
decreased blood uric acid level / MGI
increased neuron number / MGI
increased basal metabolism / MGI
abnormal neuron differentiation / MGI
decreased total body fat amount / MGI
abnormal bone mineral density / MGI
increased susceptibility to weight loss / MGI
aorta stenosis / MGI
abnormal Q wave / MGI
perinatal lethality, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
preweaning lethality, incomplete penetrance / MGI
decreased food intake / MGI
enlarged floor plate / MGI
abnormal somite border morphology / MGI
decreased basal metabolism / MGI

Literature references

Activity of the mouse Notch ligand DLL1 is sensitive to C-terminal tagging in vivo.;Schuster-Gossler Karin, Boldt Karsten, Bornhorst Dorothee, Delany-Heiken Patricia, Ueffing Marius, Gossler Achim, ;2021;BMC research notes;14;383; 34583743

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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The information provided on this page is, to the best of EMMA’s knowledge, based on data supplied by the original provider. End users are responsible for reviewing these details and for validating the strain and its suitability for their experimental use.
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STOCK Dll1em1.2Gos/Orl