C57BL/6N-Ap4e1tm1b(KOMP)Wtsi/WtsiH

Status

Only small colony available

EMMA IDEM:14331
International strain nameC57BL/6N-Ap4e1tm1b(KOMP)Wtsi/WtsiH
Alternative nameEPD0025_5_A08
Strain typeTargeted Mutant Strains
Allele/Transgene symbolAp4e1tm1b(KOMP)Wtsi,
Gene/Transgene symbolAp4e1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from KOMP ES clone EPD0025_5_A08. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using a cell permeable HTN-Cre as described in doi:10.1007/s11248-013-9764-x. Click here for more information on KOMP final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

    • Severe intellectual disability and progressive spastic paraplegia / Orphanet_280763
IMPC phenotypes (allele matching)
  • kyphosis / IMPC
  • decreased circulating LDL cholesterol level / IMPC
  • decreased circulating HDL cholesterol level / IMPC
  • abnormal heart morphology / IMPC
  • enlarged heart / IMPC
  • abnormal spleen morphology / IMPC
  • enlarged spleen / IMPC
  • enlarged lymph nodes / IMPC
  • abnormal lens morphology / IMPC
  • hyperactivity / IMPC
  • increased startle reflex / IMPC
  • limb grasping / IMPC
  • abnormal sensory capabilities/reflexes/nociception / IMPC
  • abnormal kidney morphology / IMPC
  • decreased circulating triglyceride level / IMPC
  • abnormal vitreous body morphology / IMPC
  • decreased circulating insulin level / IMPC
  • decreased vertical activity / IMPC
  • short tibia / IMPC
  • increased heart weight / IMPC
  • decreased heart weight / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • increased coping response / IMPC
  • increased erythrocyte cell number / IMPC
  • decreased cardiac output / IMPC
  • abnormal retinal inner nuclear layer morphology / IMPC
  • abnormal bone structure / IMPC
  • increased kidney weight / IMPC
  • abnormal heart left ventricle morphology / IMPC
  • increased lean body mass / IMPC
  • decreased lean body mass / IMPC
  • abnormal spine curvature / IMPC
  • long tibia / IMPC
  • abnormal behavior / IMPC
  • decreased circulating cholesterol level / IMPC
  • impaired glucose tolerance / IMPC
  • decreased heart rate / IMPC
  • decreased circulating glucose level / IMPC
  • increased cardiac muscle contractility / IMPC
  • increased prepulse inhibition / IMPC
  • decreased total body fat amount / IMPC
  • decreased grip strength / IMPC
  • increased bone mineral content / IMPC
  • decreased bone mineral content / IMPC
  • decreased respiratory quotient / IMPC
  • abnormal behavioral response to light / IMPC
  • abnormal sleep behavior / IMPC
  • increased fluid intake / IMPC
  • decreased cardiac stroke volume / IMPC
  • decreased total retina thickness / IMPC
  • increased or absent threshold for auditory brainstem response / IMPC

Order (limited)

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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