C57BL/6N-Smg9tm1b(EUCOMM)Wtsi/WtsiH

Status

Only small colony available

EMMA IDEM:14340
International strain nameC57BL/6N-Smg9tm1b(EUCOMM)Wtsi/WtsiH
Alternative nameEPD0428_2_D07
Strain typeTargeted Mutant Strains
Allele/Transgene symbolSmg9tm1b(EUCOMM)Wtsi
Gene/Transgene symbolSmg9
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0428_2_D07. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using a cell permeable HTN-Cre as described in doi:10.1007/s11248-013-9764-x. Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)
  • preweaning lethality, complete penetrance / IMPC
MGI phenotypes (allele matching)
  • lordosis / MGI
  • abnormal interatrial septum morphology / MGI
  • double outlet right ventricle / MGI
  • abnormal atrioventricular cushion morphology / MGI
  • absent kidney / MGI
  • polydactyly / MGI
  • enlarged liver sinusoidal spaces / MGI
  • abnormal pineal gland morphology / MGI
  • exencephaly / MGI
  • absent superior cervical ganglion / MGI
  • small superior cervical ganglion / MGI
  • anophthalmia / MGI
  • abnormal lens morphology / MGI
  • abnormal nasal cavity morphology / MGI
  • abnormal vein morphology / MGI
  • aphakia / MGI
  • liver cysts / MGI
  • aorta coarctation / MGI
  • abnormal eye muscle morphology / MGI
  • atrium hypoplasia / MGI
  • abnormal neurohypophysis morphology / MGI
  • abnormal optic cup morphology / MGI
  • basisphenoid bone foramen / MGI
  • fusion of vertebral bodies / MGI
  • abnormal middle ear ossicle morphology / MGI
  • coloboma / MGI
  • abnormal endolymphatic duct morphology / MGI
  • abnormal inferior vena cava morphology / MGI
  • abnormal heart position or orientation / MGI
  • abnormal cervical rib / MGI
  • thoracoschisis / MGI
  • abnormal optic chiasm morphology / MGI
  • absent optic chiasm / MGI
  • atrioventricular septal defect / MGI
  • perimembraneous ventricular septal defect / MGI
  • muscular ventricular septal defect / MGI
  • abnormal inferior vena cava valve morphology / MGI
  • persistent right dorsal aorta / MGI
  • left atrium hypoplasia / MGI
  • double ureter / MGI
  • interrupted aortic arch, type b / MGI
  • abnormal hypoglossal nerve topology / MGI
  • absent segment of posterior cerebral artery / MGI
  • subcutaneous edema / MGI
  • absent ductus venosus valve / MGI
  • abnormal ductus venosus valve morphology / MGI
  • duplication of ductus venosus / MGI
  • persistent right 6th pharyngeal arch artery / MGI
  • reduced sympathetic cervical ganglion size / MGI
  • absent connection between subcutaneous lymph vessels and lymph sac / MGI
  • blood in lymph vessels / MGI
  • heterochrony / MGI

Order (limited)

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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