C57BL/6N-Prkab1tm1b(KOMP)Wtsi/WtsiPh

Status

Available to order

EMMA IDEM:14536
International strain nameC57BL/6N-Prkab1tm1b(KOMP)Wtsi/WtsiPh
Alternative nameEPD0033_3_C09
Strain typeTargeted Mutant Strains
Allele/Transgene symbolPrkab1tm1b(KOMP)Wtsi,
Gene/Transgene symbolPrkab1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from KOMP ES clone EPD0033_3_C09. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using a cell permeable HTN-Cre as described in doi:10.1007/s11248-013-9764-x. Click here for more information on KOMP final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitute of Molecular Genetics, Prague, Czech Republic

Disease and phenotype information

IMPC phenotypes (allele matching)
  • increased bone mineral density / IMPC
  • kyphosis / IMPC
  • decreased circulating LDL cholesterol level / IMPC
  • decreased circulating HDL cholesterol level / IMPC
  • decreased hematocrit / IMPC
  • enlarged heart / IMPC
  • abnormal spleen morphology / IMPC
  • enlarged spleen / IMPC
  • decreased body length / IMPC
  • anophthalmia / IMPC
  • hypoactivity / IMPC
  • decreased caudal vertebrae number / IMPC
  • decreased mean corpuscular volume / IMPC
  • increased mean platelet volume / IMPC
  • abnormal retinal vasculature morphology / IMPC
  • decreased hemoglobin content / IMPC
  • decreased erythrocyte cell number / IMPC
  • decreased lean body mass / IMPC
  • decreased circulating iron level / IMPC
  • abnormal spine curvature / IMPC
  • abnormal behavior / IMPC
  • increased spleen weight / IMPC
  • decreased circulating cholesterol level / IMPC
  • impaired glucose tolerance / IMPC
  • increased circulating bilirubin level / IMPC
  • decreased mean corpuscular hemoglobin / IMPC
  • decreased mean corpuscular hemoglobin concentration / IMPC
  • increased glycosylated hemoglobin level / IMPC
  • decreased circulating amylase level / IMPC
  • decreased total body fat amount / IMPC
  • increased grip strength / IMPC
  • increased red blood cell distribution width / IMPC
  • abnormal retinal blood vessel morphology / IMPC
  • shortened RR interval / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
  • increased fluid intake / IMPC
  • decreased total retina thickness / IMPC
  • increased memory-marker gamma-delta T cell number / IMPC
  • increased memory-marker CD4-negative NK T cell number / IMPC
MGI phenotypes (allele matching)
  • decreased hematocrit / MGI
  • extramedullary hematopoiesis / MGI
  • abnormal erythropoiesis / MGI
  • echinocytosis / MGI
  • schistocytosis / MGI
  • enlarged spleen / MGI
  • hemolytic anemia / MGI
  • abnormal bone marrow morphology / MGI
  • abnormal erythrocyte morphology / MGI
  • increased mean platelet volume / MGI
  • reticulocytosis / MGI
  • anisocytosis / MGI
  • microcytic anemia / MGI
  • microcytosis / MGI
  • decreased hemoglobin content / MGI
  • decreased erythrocyte cell number / MGI
  • increased erythroid progenitor cell number / MGI
  • abnormal erythrocyte osmotic lysis / MGI
  • decreased circulating iron level / MGI
  • increased spleen weight / MGI
  • decreased mean corpuscular hemoglobin concentration / MGI
  • increased spleen red pulp amount / MGI
  • increased spleen iron level / MGI
  • increased red blood cell distribution width / MGI
  • stomatocytosis / MGI
  • acanthocytosis / MGI
  • increased erythroblast number / MGI
  • increased circulating erythropoietin level / MGI
  • increased circulating ferritin level / MGI
  • abnormal reticulocyte cell number / MGI

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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