C57BL/6N-Del(6Tmem176b-Tmem176a)1Ced/Orl
| Status | Available to order |
| EMMA ID | EM:14682 |
| Citation information | RRID:IMSR_EM:14682 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6N-Del(6Tmem176b-Tmem176a)1Ced/Orl |
| Alternative name | Tmem176a/b DKO |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Del(6Tmem176b-Tmem176a)1Ced |
| Gene/Transgene symbol | Del(6Tmem176b-Tmem176a)1Ced |
Information from provider
| Provider | Cedric Louvet |
| Provider affiliation | CRTI UMR1064 |
| Genetic information | Endonuclease-mediated mutation of Tmem176a and Tmem176b (double KO, DKO), located within the same genomic locus in opposite directions. The first coding exon of each gene was targeted using two CRISPR gRNA. An allele bearing a deletion between these two sites (ca. 4.2 kb) was selected. Allele: Del(6Tmem176b-Tmem176a)1Ced (MGI:6828965). Strain: C57BL/6N-Del(6Tmem176b-Tmem176a)1Ced/Ced (MGI:6828978). CRISPR technology details: the Cas9 expression vector from Addgene (plasmid #43861) was used and the Cas9 coding sequence has not been integrated in the mouse genome (microinjected in the embryos in the form of RNA). |
| Phenotypic information | Homozygous:Reduced ability of dendritic cells to present exogenous antigen to CD4+ T cells through MHC-II.Heterozygous:No phenotype described |
| Breeding history | 11 backcrosses onto the C57BL/6NCrl background were performed from the F0 founder mouse (generated in the C57BL/6NRj (Janvier Lab) background). |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | homozygous C57BL/6N males |
Literature references
- Dendritic Cells Require TMEM176A/B Ion Channels for Optimal MHC Class II Antigen Presentation to Naive CD4+ T Cells.;Lancien Melanie, Bienvenu Geraldine, Salle Sonia, Gueno Lucile, Feyeux Magalie, Merieau Emmanuel, Remy Severine, Even Amandine, Moreau Aurelie, Molle Alice, Fourgeux Cynthia, Coulon Flora, Beriou Gaelle, Bouchet-Delbos Laurence, Chiffoleau Elise, Kirstetter Peggy, Chan Susan, Kerfoot Steven M, Abdu Rahiman Saeed, De Simone Veronica, Matteoli Gianluca, Boncompain Gaelle, Perez Franck, Josien Regis, Poschmann Jeremie, Cuturi Maria Cristina, Louvet Cedric, ;2021;Journal of immunology (Baltimore, Md. : 1950);207;421-435; 34233909
- Generation of a Double KO Mouse by Simultaneous Targeting of the Neighboring Genes Tmem176a and Tmem176b Using CRISPR/Cas9: Key Steps from Design to Genotyping.;Lemoine Aurélie, Chauveau-Le Friec Gaëlle, Langa Francina, Louvet Cédric, ;2016;Journal of genetics and genomics = Yi chuan xue bao;43;329-40; 27234594