RjHan:SD rat CST3

Status	Available to order
EMMA ID	EM:14763
Citation information	EM:14763
International strain name	RjHan:SD rat CST3
Alternative name	rCST3
Strain type	Endonuclease-mediated
Allele/Transgene symbol	rat Cst3^M88Q
Gene/Transgene symbol	rat Cst3

Information from provider

Provider	Jana Kopkanova
Provider affiliation	Transgenic and Archiving Unit, Institute of Molecular Genetics of the Czech Academy of Sciences
Genetic information	cystatin C (Cst3, CYSC, MGC105556) gene; mutant rats were generated by zygote electroporation of gene-specific gRNA (5´-CTATTATTTGGATGTGGAGA-3´), Cas9 and ssODN (5´-CAGTTAGTCAAATTTGTCTGGGACTTGGTACATGTAGTTCGGCCCtgCTCCA CgTCCAAATAATAGTTTATTCCAGCCACAAGCTACAGTGAAAAAGAG-3´) template was used to introduce M88Q (p.Met88Gln) point mutation into the 2nd coding exon. Positive founders were identified by RFLP analysis.
Phenotypic information	Homozygous: not analyzed Heterozygous: not analyzed
Breeding history	2 generations crossed to RjHan:SD background
References	None available
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Institute of Molecular Genetics, Prague, Czech Republic
Animals used for archiving	wild-type rat Sprague-Dawley males, heterozygous rat Sprague-Dawley females
Stage of embryos	2-cell

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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