C57BL/6-Ankrd31em3Flor/Orl
| Status | Available to order |
| EMMA ID | EM:14779 |
| Citation information | RRID:IMSR_EM:14779 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Ankrd31em3Flor/Orl |
| Alternative name | ANKRD31 |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Ankrd31em3Flor |
| Gene/Transgene symbol | Ankrd31 |
Information from provider
| Provider | Florian GUILLOU |
| Provider affiliation | INRAE |
| Genetic information | Ankrd31 knock-out male mouse model (Ankrd31-/-). Ankrd31 mutant lines were generated using CRISPR/Cas9 genome editing. Our strategy used two guide RNAs for the deletion of exon 4 and generated a frameshift to create a stop codon. Line 432: the two guide RNAs slightly slipped, the cuts made by CRISPR/Cas9 shifted at 5’ by 4 nucleotides upstream of the theoretical cut-off site and at 3’ by 14 nucleotides downstream of the theoretical cut-off site. This caused a change in reading frame and the stop codon was shifted by 15 nucleotides. In this line of mice, a residual peptide of 66 amino acids added with 5 amino acids without homology with ANKRD31 protein should be expressed. CRISPR technology details: the Cas9 protein was obtained from PNA Bio. |
| Phenotypic information | Homozygous:Ankrd31 -/- male mice were infertile exhibiting oligo-astheno-teratozoospermia (a low number of immotile spermatozoa with abnormal morphological features). In addition, a complete deregulation of the blood-epididymal barrier was found in Ankrd31-/- mice due to cell-to-cell junction anomalies. Females Ankrd -/- were fertile.Heterozygous:Heterozygous males were fertile. |
| Breeding history | Ninety injected embryos with the two guide RNAs were transferred into females and 14 mice were born. Eleven of the 14 born pups had alterations in the targeted genomic locus. Eight mice showed a single CRISPR/Cas9 cut-off site. Only three mice showed the double cleavage leading to the deletion of exon 4. Homozygote Ankrd31 gene deletion affects male fertility. Females homozygous for the Ankrd31 gene deletion were fertile. |
| References |
|
| Homozygous fertile | females only |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J males |
Literature references
- Ankrd31 in Sperm and Epididymal Integrity.;Manfrevola Francesco, Martinez Guillaume, Coutton Charles, Rocco Domenico, Reynaud Karine, Le Vern Yves, Froment Pascal, Beauclair Linda, Aubert Denise, Pierantoni Riccardo, Chianese Rosanna, Guillou Florian, ;2021;Frontiers in cell and developmental biology;9;741975; 34820371