Sprague Dawley rat Crl:CD(SD)-KO(Gnal*1)44Hpn/Hpn
| Status | Available to order |
| EMMA ID | EM:14794 |
| Citation information | EM:14794 |
| International strain name | Sprague Dawley rat Crl:CD(SD)-KO(Gnal*1)44Hpn/Hpn |
| Alternative name | Crl:CD(SD)-KO(Gnal*1)44Hpn/Hpn |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | rat Gnalspl-variant 2-del44 |
| Gene/Transgene symbol | rat Gnal |
Information from provider
| Provider | Huu Phuc Nguyen |
| Provider affiliation | Institute of Medical Genetics and Applied Genomics, University of Tuebingen |
| Genetic information | Gnal knockout rats were generated by CRISPR/Cas9 technology. Exon1 of rat Gnal splicing variant 2 was targeted, resulting in a deletion of 1 base pair that corresponds to position 44 downstream of the translation start point ATG of the Gnal splicing variant 2. CRISPR technology details: self-synthesized Cas9 mRNA using px 330 vector, applying T7 transcription kit followed by Poly-Adenylation and Capping. No sequencing performed, whether Cas9 has been inserted into the rat genome is unknown. |
| Phenotypic information | Homozygous:Approximately 25% body weight reduction; Smaller body size; Hyperactivity; Better rotarod performance than wild-type littermates. Heterozygous:No body weight change; No body size change; Hypo-activity; Worse rotarod performance than wild-type littermates. |
| Breeding history | Outbreeding for approximately 16 generations |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous rat Sprague-Dawley males, wild-type rat Sprague-Dawley females |
| Stage of embryos | 2-cell |